Release of renal dipeptidase from rabbit renal proximal tubules and its inhibition by gentamicin
| Autor: | Jeoung Soon We, Kyong Choi, Hwanghee Blaise Lee, Bok Yun Kang, Haeng Soon Park, Ho Jae Han |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Dipeptidases
Lipopolysaccharide Blotting Western Biology Pharmacology Kidney Nephrotoxicity Kidney Tubules Proximal chemistry.chemical_compound Drug Discovery medicine Animals Protease Inhibitors Protein Synthesis Inhibitors Cisplatin Organic Chemistry Temperature Rabbit (nuclear engineering) Renal dipeptidase Blot medicine.anatomical_structure chemistry Biochemistry Molecular Medicine Proximal tubule Gentamicin Rabbits Gentamicins medicine.drug |
| Zdroj: | Archives of Pharmacal Research. 22:367-371 |
| ISSN: | 1976-3786 0253-6269 |
| Popis: | Effects of several drugs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Western blotting. RDPase was also released from cisplatin, lipopolysaccharide (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way. |
| Databáze: | OpenAIRE |
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