Identification of a Novel A-kinase Anchoring Protein 18 Isoform and Evidence for Its Role in the Vasopressin-induced Aquaporin-2 Shuttle in Renal Principal Cells
Autor: | Volker Henn, Friedrich W. Herberg, Michael Beyermann, Eduard Stefan, Walter Rosenthal, Burkhard Wiesner, Giovana Valenti, Sebastian Bachmann, Roland Schmitt, Dorothea Lorenz, Enno Klussmann, Eberhard Krause, Grazia Tamma, Franziska Theilig, Bayram Edemir, Lutz Vossebein |
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Rok vydání: | 2004 |
Předmět: |
A-kinase-anchoring protein
Vasopressin Time Factors Arginine Protein Conformation A Kinase Anchor Proteins Kidney urologic and male genital diseases Biochemistry Cyclic AMP Fluorescence Resonance Energy Transfer Protein Isoforms Cloning Molecular Receptor Cells Cultured Glutathione Transferase Kidney Medulla Immunohistochemistry Cell biology Protein Transport medicine.anatomical_structure Aquaporin 2 Intracellular Protein Binding Subcellular Fractions DNA Complementary Vasopressins Recombinant Fusion Proteins Blotting Western Biology Aquaporins Cell Line medicine Animals Humans RNA Messenger Protein kinase A Molecular Biology Adaptor Proteins Signal Transducing Gene Library urogenital system Cell Membrane Membrane Proteins Cell Biology Surface Plasmon Resonance Blotting Northern Precipitin Tests Rats Arginine Vasopressin Enzyme Activation Kinetics RNA Carrier Proteins |
Zdroj: | Journal of Biological Chemistry. 279:26654-26665 |
ISSN: | 0021-9258 |
Popis: | Arginine vasopressin (AVP) increases the water permeability of renal collecting duct principal cells by inducing the fusion of vesicles containing the water channel aquaporin-2 (AQP2) with the plasma membrane (AQP2 shuttle). This event is initiated by activation of vasopressin V2 receptors, followed by an elevation of cAMP and the activation of protein kinase A (PKA). The tethering of PKA to subcellular compartments by protein kinase A anchoring proteins (AKAPs) is a prerequisite for the AQP2 shuttle. During the search for AKAP(s) involved in the shuttle, a new splice variant of AKAP18, AKAP18delta, was identified. AKAP18delta functions as an AKAP in vitro and in vivo. In the kidney, it is mainly expressed in principal cells of the inner medullary collecting duct, closely resembling the distribution of AQP2. It is present in both the soluble and particulate fractions derived from renal inner medullary tissue. Within the particulate fraction, AKAP18delta was identified on the same intracellular vesicles as AQP2 and PKA. AVP not only recruited AQP2, but also AKAP18delta to the plasma membrane. The elevation of cAMP caused the dissociation of AKAP18delta and PKA. The data suggest that AKAP18delta is involved in the AQP2 shuttle. |
Databáze: | OpenAIRE |
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