Acute toxicity of functionalized single wall carbon nanotubes: A biochemical, histopathologic and proteomics approach
Autor: | Homa Ahmadi, Kamal Razavi Azarkhiavi, Ahad Mokhtarzadeh, Azadeh Hashem Nia, Khalil Abnous, Mohammad Ramezani, Maryam Matbou Riahi, Behzad Behnam, Bibi Marjan Razavi, Rezvan Yazdian-Robati |
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Rok vydání: | 2017 |
Předmět: |
Proteomics
0301 basic medicine Antioxidant medicine.medical_treatment Polysorbates Selenium-Binding Proteins 02 engineering and technology Toxicology Polyethylene Glycols Mice 03 medical and health sciences chemistry.chemical_compound Western blot Malondialdehyde medicine Animals Electrophoresis Gel Two-Dimensional Aspartate Aminotransferases HSP90 Heat-Shock Proteins Selenium binding Mice Inbred BALB C medicine.diagnostic_test Nanotubes Carbon Alanine Transaminase Methyltransferases General Medicine Glutathione 021001 nanoscience & nanotechnology Regucalcin Acute toxicity Oxidative Stress 030104 developmental biology Liver chemistry Biochemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization GNMT Toxicity 0210 nano-technology Peroxiredoxin VI |
Zdroj: | Chemico-Biological Interactions. 275:196-209 |
ISSN: | 0009-2797 |
Popis: | Recently carbon nanotubes (CNTs) showed promising potentials in different biomedical applications but their safe use in humans and probable toxicities are still challenging. The aim of this study was to determine the acute toxicity of functionalized single walled carbon nanotubes (SWCNTs). In this project, PEGylated and Tween functionalized SWCNTs were prepared. BALB/c mice were randomly divided into nine groups, including PEGylated SWCNTs (75,150μg/mouse) and PEG, Tween80 suspended SWCNTs, Tween 80 and a control group (intact mice). One or 7 days after intravenous injection, the mice were killed and serum and livers were collected. The oxidative stress markers, biochemical and histopathological changes were studied. Subsequently, proteomics approach was used to investigate the alterations of protein expression profiles in the liver. Results showed that there were not any significant differences in malondealdehyde (MDA), glutathione (GSH) levels and biochemical enzymes (ALT and AST) between groups, while the histopathological observations of livers showed some injuries. The results of proteomics analysis revealed indolethylamine N-Methyltransferase (INMT), glycine N-Methyltransferase (GNMT), selenium binding protein (Selenbp), thioredoxin peroxidase (TPx), TNF receptor associated protein 1(Trap1), peroxiredoxin-6 (Prdx6), electron transport flavoprotein (Etf-α), regucalcin (Rgn) and ATP5b proteins were differentially expressed in functionalized SWCNTs groups. Western blot analyses confirmed that the changes in Prdx6 were consistent with 2-DE gel analysis. In summary, acute toxicological study on two functionalized SWCNTs did not show any significant toxicity at selected doses. Proteomics analysis also showed that following exposure to functionalized SWCNTs, the expression of some proteins with antioxidant activity and detoxifying properties were increased in liver tissue. |
Databáze: | OpenAIRE |
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