Ex vivo tumor necrosis factor-alpha response of blood leukocytes in Danish Holstein-Friesian cows stimulated by Gram-positive and Gram-negative bacteria isolated from mastitic milk
| Autor: | C.M. Røntved, Jens Bech Andersen, Birthe Marie Damgaard, M. Khatun |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Denmark
Antibiotics medicine.disease_cause Tumor Necrosis Factor-alpha/analysis BOVINE MASTITIS 0403 veterinary science Gram-Negative Bacteria/classification Leukocytes Leukocytes/immunology Mastitis Bovine DAIRY-COWS 0303 health sciences biology Gram-Positive Bacteria/classification 04 agricultural and veterinary sciences TNF-ALPHA Early lactation STREPTOCOCCUS-DYSGALACTIAE Ex vivo whole blood assay Parity Milk Staphylococcus aureus Female Gram-negative bacteria Mastitis Bovine/microbiology 040301 veterinary sciences medicine.drug_class Immunology Cattle Diseases Gram-Positive Bacteria Microbiology 03 medical and health sciences In vivo Gram-Negative Bacteria WHOLE-BLOOD INTRAMAMMARY INFECTION medicine Animals Lactation Milk/microbiology STAPHYLOCOCCUS-AUREUS 030304 developmental biology Streptococcus uberis Mastitis bacteria General Veterinary Tumor Necrosis Factor-alpha Cattle Diseases/microbiology CYTOKINE PRODUCTION biology.organism_classification TNF-α Cattle Streptococcus dysgalactiae DIFFERENT PATTERNS INFLAMMATORY MEDIATORS Ex vivo Bacteria |
| Zdroj: | Khatun, M, Damgaard, B M, Andersen, J B & Røntved, C M 2021, ' Ex vivo tumor necrosis factor-α response of blood leukocytes in Danish Holstein Friesian cows stimulated by Gram-positive and Gram-negative bacteria isolated from mastitic milk ', Veterinary Immunology and Immunopathology, vol. 234, 110204 . https://doi.org/10.1016/j.vetimm.2021.110204 |
| ISSN: | 1873-2534 |
| DOI: | 10.1016/j.vetimm.2021.110204 |
| Popis: | A whole blood stimulation assay was used to investigate the effects of parity, number of weeks after calving and Gram-positive and Gram-negative bacteria on the ex vivo TNF-α responsiveness of Danish Holstein-Friesian cows of first to third lactation (n = 28). Blood samples were collected in weeks 2, 3, 5 and 8 after parturition and stimulated with Escherichia coli LPS (10 μg/mL), Staphylococcus aureus peptidoglycan (PGN, 10 μg/mL) and dead Escherichia coli, Streptococcus uberis, Staphylococcus aureus, and Streptococcus dysgalactiae at a concentration of 2.5 × 10 6/mL. The antibiotic polymyxin-B (100 μg/mL) was added to the Gram-positive bacteria to avoid the influence of environmental endotoxin by ELISA test. Overall, parity had no effect, whereas number of weeks after calving altered the TNF-α responsiveness of the majority of the stimulants. Ex vivo, Gram-positive bacteria always resulted in a higher TNF-α response than Gram-negative bacteria with large differences within the individual cows. High correlations were found within the Gram-negative stimulants panel (r = 0.83) and within the Gram-positive (r = 0.81 to 0.86) stimulants panel except PGN. The higher TNF-α responsiveness by Gram-positive bacteria is in agreement with in vitro studies in human but in contrast to the in vivo TNF-α responsiveness in bovine udder. |
| Databáze: | OpenAIRE |
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