Radiation-Induced Lung Adenocarcinoma is Associated with Increased Frequency of Genes Inactivated by Promoter Hypermethylation
| Autor: | Frank D. Gilliland, Frederick D. Gentry, Thomas H. March, Donna M. Klinge, Terri Kang, Christopher M. Lyon, Galina Adamova, Steven A. Belinsky, Vitaliy Telnov, G. G. Rusinova, Kieu C. Liechty |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
Male Lung Neoplasms Neoplasms Radiation-Induced GATA5 Transcription Factor Biophysics Adenocarcinoma Biology CDKN2A Occupational Exposure medicine Humans Gene silencing Radiology Nuclear Medicine and imaging Epigenetics Promoter Regions Genetic Lung cancer Aged Radiation PAX5 Transcription Factor Cancer Methylation DNA Methylation Middle Aged Cadherins medicine.disease Molecular biology DNA methylation Cancer research Female DNA Damage |
| Zdroj: | Radiation Research. 168:409-414 |
| ISSN: | 1938-5404 0033-7587 |
| Popis: | Epigenetic inactivation of genes by promoter hypermethylation, a major mechanism in the initiation and progression of tobacco-induced cancer, has also been associated with lung cancer induced through environmental and occupational exposures. Our previous study of gene methylation in workers from the MAYAK nuclear enterprise identified a significantly higher prevalence for methylation of the p16 gene (CDKN2A) in adenocarcinomas from workers compared to tumors from non-worker controls. The purpose of this investigation was to determine whether genes in addition to p16 are "targeted" for silencing and whether overall gene methylation was more common in radiation-induced adenocarcinoma. A significant increase in the prevalence of methylation of GATA5 was seen in tumors from workers compared to tumors from controls. The prevalence for methylation of PAX5 beta and H-cadherin did not differ in tumors from workers and controls. Evaluating the frequency for methylation of a five-gene panel revealed that 93% of adenocarcinomas from workers compared to 66% of tumors from controls were methylated for at least one gene. Moreover, a twofold increase was seen in the number of tumors methylated for three or more genes for tumors from workers compared to controls. Increased frequency for inactivation of genes by promoter hypermethylation and targeting of tumor suppressor genes such as GATA5 may be factors that contribute to the increased risk for lung cancer associated with radiation exposure. |
| Databáze: | OpenAIRE |
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