Comparative Pathomorphological, Mycological and Molecular Examination of Turkey Poults with Different Immunological Status Experimentally Infected with Aspergillus fumigatus
Autor: | Oliver Radanovic, Nemanja Jezdimirović, Jadranka Žutić, Ljiljana Spalević, Vojin Ivetic, Branislav Kureljušić, Dejan Krnjaić, Milijan Jovanović |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Veterinary medicine respiratory organs 030106 microbiology Aspergillus fumigatus Microbiology nested pcr 03 medical and health sciences Immune system SF600-1100 medicine Respiratory system Dexamethasone turkey poults General Veterinary biology Inoculation aspergillus fumigatus biology.organism_classification 3. Good health Spore nested PCR 030104 developmental biology Histopathology Nested polymerase chain reaction medicine.drug |
Zdroj: | Acta Veterinaria-Beograd Acta Veterinaria, Vol 69, Iss 2, Pp 201-217 (2019) |
ISSN: | 1820-7448 |
Popis: | The aim of this study was to determine the pathological, mycological and molecular findings in turkey poults with different immunological status experimentally infected with Aspergillus fumigatus. The investigation was carried out 1, 3, 7, 14 and 21 days after intratracheal inoculation of 5.056×107 spores of A. fumigatus to 14-day-old turkey poults in group G-1, as well as to turkey poults in group G-2 which were treated prior to infection with dexamethasone. A. fumigatus was isolated on day 1 p.i. in both groups, but the number of positive samples was bigger in group G-1. A. fumigatus was isolated from the respiratory organs of group G-1as early as on day 1 and 3 p.i. in 4 out of 12 examined specimens (33%). On day 7 p.i. A. fumigatus was possible to isolate from the respiratory organs of 50% of infected birds, on day 14 in 83.33% and on day 21 p.i. A. fumigatus was isolated in 6 out of 6 sacrificed turkey poults (100%). In dexamethasone-treated group A. fumigatus isolates from the respiratory organs on day 1 and 3 p.i. were same as in group G-1, whereas on days 7 and 14 p.i. the number of turkey poults positive to A. fumigatus increased in comparison with the untreated G-1 group. The histopathological lesions in turkey poults treated with dexamethasone developed earlier, were more intensive and extensive. The mycological and nested PCR results revealed a higher number of samples positive for the presence of A. fumigatus DNA in the group G-2, pretreated with dexamethasone. |
Databáze: | OpenAIRE |
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