Involvement of CD24 in angiogenesis in a mouse model of oxygen-induced retinopathy

Autor:	Hadas Newman, Anat Loewenstein, Nadir Arber, Mordechai Rosner, Sarah Pri-Chen, Oriel Spierer, Adiel Barak, Shiran Shapira, Sarah Kraus
Rok vydání:	2012
Předmět:	medicine.medical_specialty Angiogenesis Hyperoxia Retinal Neovascularization Neovascularization Cellular and Molecular Neuroscience chemistry.chemical_compound Mice Ophthalmology medicine Animals Humans Retinopathy of Prematurity Oxygen toxicity Mice Knockout business.industry Infant Newborn CD24 Antigen Retinal Vessels Retinopathy of prematurity Retinal Dextrans medicine.disease Fluoresceins Sensory Systems Mice Inbred C57BL Oxygen Disease Models Animal medicine.anatomical_structure chemistry Animals Newborn medicine.symptom business Perfusion Blood vessel Retinopathy
Zdroj:	Current eye research. 37(6)
ISSN:	1460-2202
Popis:	To investigate a possible involvement of CD24 in vascular remodeling and angiogenesis in retinopathy of prematurity (ROP) in a mouse model of oxygen-induced retinopathy.17 CD24 knockout (KO) and 12 wild-type (WT) C57BL/6 mice were used. Group 1 mice were exposed to oxygen concentrations of 75 ± 2% from postnatal day (P) 7 to P12. Group 2 mice were raised in room air. At P17, all mice underwent fluorescein-conjugated-dextran perfusion and were sacrificed. The flat-mounted retinas were scored manually and digitally by a new computerized algorithm, according to blood vessel obliteration, tortuosity, vascular tufts and neovascularization formation.Fifty four retinal whole mounts were available for analysis and scoring. Group 1 retinas had significantly higher values of vaso-obliteration, tufts, neovascularization, vessel tortuosity and higher mean retinopathy scores than Group 2 retinas (KO mice: 9.0 ± 0.27 vs. 0.74 ± 0.2, respectively, P 0.0001; WT mice: 7.58 ± 0.40 vs. 1.17 ± 0.27, respectively, P 0.0001). Manual scoring in Group 1 revealed higher values of neovascularization, tortuosity and mean retinopathy scores in KO mice vs. WT mice (9.0 ± 0.27 vs. 7.58 ± 0.40, respectively, P = 0.009). Digital scoring revealed a higher neovascularization score in KO mice as well (13.72 ± 0.82% vs. 8.06 ± 0.27%, P 0.0001). All mice had similar vaso-obliteration areas. There were no significant differences between KO and WT mice in Group 2.Absence of CD24 may have a deleterious effect on angiogenesis occurring in the second stage of ROP development, though its role in vessel obliteration during the first stage of ROP is probably limited.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8757feea984c1caf12dec17f4080ec8d https://pubmed.ncbi.nlm.nih.gov/22577772 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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