High-resolution nuclear magnetic resonance spectroscopy in microfluidic droplets
| Autor: | William G. Hale, Marcel Utz, Graeme Finch, Gabriel Rossetto, Rachael Greenhalgh |
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| Rok vydání: | 2018 |
| Předmět: |
Materials science
Proton business.industry Microfluidics Biomedical Engineering Resonance Bioengineering 02 engineering and technology General Chemistry Nuclear magnetic resonance spectroscopy 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Magnetic susceptibility 0104 chemical sciences Physics::Fluid Dynamics NMR spectra database Oil droplet Phase (matter) Optoelectronics Condensed Matter::Strongly Correlated Electrons 0210 nano-technology business |
| Zdroj: | Lab on a Chip |
| ISSN: | 1473-0189 |
| Popis: | A generic approach is presented that allows high-resolution NMR spectroscopy of water/oil droplet emulsions in microfluidic devices. Microfluidic NMR spectroscopy has recently made significant advances due to the design of micro-detector systems and their successful integration with microfluidic devices. Obtaining NMR spectra of droplet suspensions, however, is complicated by the inevitable differences in magnetic susceptibility between the chip material, the continuous phase, and the droplet phases. This leads to broadening of the NMR resonance lines and results in loss of spectral resolution. We have mitigated the susceptibility difference between the continuous (oil) phase and the chip material by incorporating appropriately designed air-filled structures into the chip. The susceptibilities of the continuous and droplet (aqueous) phases have been matched by doping the droplet phase with a Eu3+ complex. Our results demonstrate that this leads to a proton line width in the droplet phase of about 3 Hz, enabling high-resolution NMR techniques. |
| Databáze: | OpenAIRE |
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