Intracisternal delivery of PEG-coated gold nanoparticles results in high brain penetrance and long-lasting stability
Autor: | Pierfausto Seneci, Daniela Arosio, Andrea Menegon, Laura Polito, Maria Girelli, Gianvito Martino, Paola Podini, Luca Muzio, Antonello E. Spinelli |
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Přispěvatelé: | Spinelli, A., Girelli, M., Arosio, D., Polito, L., Podini, P., Martino, G., Seneci, P., Muzio, L., Menegon, A. |
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Genetic enhancement
Cell Metal Nanoparticles Pharmaceutical Science Medicine (miscellaneous) 02 engineering and technology Cisterna magna 01 natural sciences Applied Microbiology and Biotechnology Polyethylene Glycols Mice Drug Stability Tissue Distribution Neurons medicine.diagnostic_test Chemistry Brain Carbocyanines 021001 nanoscience & nanotechnology Intra cisterna magna Cell biology medicine.anatomical_structure lcsh:R855-855.5 Colloidal gold Molecular Medicine 0210 nano-technology lcsh:Medical technology Cell Survival lcsh:Biotechnology Biomedical Engineering Bioengineering 010402 general chemistry Immunofluorescence Permeability Cell Line In vivo analysis In vivo lcsh:TP248.13-248.65 Cisterna Magna Parenchyma medicine Animals Humans Distribution (pharmacology) Gold nanoparticles Fluorescent Dyes Research 0104 chemical sciences Gold |
Zdroj: | Journal of nanobiotechnology 17 (2019). doi:10.1186/s12951-019-0481-3 info:cnr-pdr/source/autori:Spinelli, Antonello; Girelli, Maria; Arosio, Daniela; Polito, Laura; Podini, Paola; Martino, Gianvito; Seneci, Pierfausto; Muzio, Luca; Menegon, Andrea/titolo:Intracisternal delivery of PEG-coated gold nanoparticles results in high brain penetrance and long-lasting stability/doi:10.1186%2Fs12951-019-0481-3/rivista:Journal of nanobiotechnology/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:17 Journal of Nanobiotechnology, Vol 17, Iss 1, Pp 1-13 (2019) Journal of Nanobiotechnology |
DOI: | 10.1186/s12951-019-0481-3 |
Popis: | Background The increasing use of gold nanoparticles (AuNPs) in the field of neuroscience instilled hope for their rapid translation to the clinical practice. AuNPs can be engineered to carry therapeutics or diagnostics in the diseased brain, possibly providing greater cell specificity and low toxicity. Although there is a general enthusiasm for these tools, we are in early stages of their development. Overall, their brain penetrance, stability and cell specificity are critical issues that must be addressed to drive AuNPs to the clinic. Results We studied the kinetic, distribution and stability of PEG-coated AuNPs in mice receiving a single injection into the cisterna magna of the 4th ventricle. AuNPs were conjugated with the fluorescent tag Cy5.5 (Cy5.5-AuNPs) to track their in vivo distribution. Fluorescence levels from such particles were detected in mice for weeks. In situ analysis of brains by immunofluorescence and electron microscopy revealed that Cy5.5-AuNPs penetrated the brain parenchyma, spreading in the CNS parenchyma beneath the 4th ventricle. Cy5.5-AuNPs were preferentially found in neurons, although a subset of resting microglia also entrapped these particles. Conclusions Our results suggest that the ICM route for delivering gold particles allows the targeting of neurons. This approach might be pursued to carry therapeutics or diagnostics inside a diseased brain with a surgical procedure that is largely used in gene therapy approaches. Furthermore, this approach could be used for radiotherapy, enhancing the agent’s efficacy to kill brain cancer cells. Electronic supplementary material The online version of this article (10.1186/s12951-019-0481-3) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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