Intracisternal delivery of PEG-coated gold nanoparticles results in high brain penetrance and long-lasting stability

Autor: Pierfausto Seneci, Daniela Arosio, Andrea Menegon, Laura Polito, Maria Girelli, Gianvito Martino, Paola Podini, Luca Muzio, Antonello E. Spinelli
Přispěvatelé: Spinelli, A., Girelli, M., Arosio, D., Polito, L., Podini, P., Martino, G., Seneci, P., Muzio, L., Menegon, A.
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Genetic enhancement
Cell
Metal Nanoparticles
Pharmaceutical Science
Medicine (miscellaneous)
02 engineering and technology
Cisterna magna
01 natural sciences
Applied Microbiology and Biotechnology
Polyethylene Glycols
Mice
Drug Stability
Tissue Distribution
Neurons
medicine.diagnostic_test
Chemistry
Brain
Carbocyanines
021001 nanoscience & nanotechnology
Intra cisterna magna
Cell biology
medicine.anatomical_structure
lcsh:R855-855.5
Colloidal gold
Molecular Medicine
0210 nano-technology
lcsh:Medical technology
Cell Survival
lcsh:Biotechnology
Biomedical Engineering
Bioengineering
010402 general chemistry
Immunofluorescence
Permeability
Cell Line
In vivo analysis
In vivo
lcsh:TP248.13-248.65
Cisterna Magna
Parenchyma
medicine
Animals
Humans
Distribution (pharmacology)
Gold nanoparticles
Fluorescent Dyes
Research
0104 chemical sciences
Gold
Zdroj: Journal of nanobiotechnology 17 (2019). doi:10.1186/s12951-019-0481-3
info:cnr-pdr/source/autori:Spinelli, Antonello; Girelli, Maria; Arosio, Daniela; Polito, Laura; Podini, Paola; Martino, Gianvito; Seneci, Pierfausto; Muzio, Luca; Menegon, Andrea/titolo:Intracisternal delivery of PEG-coated gold nanoparticles results in high brain penetrance and long-lasting stability/doi:10.1186%2Fs12951-019-0481-3/rivista:Journal of nanobiotechnology/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:17
Journal of Nanobiotechnology, Vol 17, Iss 1, Pp 1-13 (2019)
Journal of Nanobiotechnology
DOI: 10.1186/s12951-019-0481-3
Popis: Background The increasing use of gold nanoparticles (AuNPs) in the field of neuroscience instilled hope for their rapid translation to the clinical practice. AuNPs can be engineered to carry therapeutics or diagnostics in the diseased brain, possibly providing greater cell specificity and low toxicity. Although there is a general enthusiasm for these tools, we are in early stages of their development. Overall, their brain penetrance, stability and cell specificity are critical issues that must be addressed to drive AuNPs to the clinic. Results We studied the kinetic, distribution and stability of PEG-coated AuNPs in mice receiving a single injection into the cisterna magna of the 4th ventricle. AuNPs were conjugated with the fluorescent tag Cy5.5 (Cy5.5-AuNPs) to track their in vivo distribution. Fluorescence levels from such particles were detected in mice for weeks. In situ analysis of brains by immunofluorescence and electron microscopy revealed that Cy5.5-AuNPs penetrated the brain parenchyma, spreading in the CNS parenchyma beneath the 4th ventricle. Cy5.5-AuNPs were preferentially found in neurons, although a subset of resting microglia also entrapped these particles. Conclusions Our results suggest that the ICM route for delivering gold particles allows the targeting of neurons. This approach might be pursued to carry therapeutics or diagnostics inside a diseased brain with a surgical procedure that is largely used in gene therapy approaches. Furthermore, this approach could be used for radiotherapy, enhancing the agent’s efficacy to kill brain cancer cells. Electronic supplementary material The online version of this article (10.1186/s12951-019-0481-3) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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