Trends and molecular characteristics of carbapenemase-producing Enterobacteriaceae in Japanese hospital from 2006 to 2015

Autor: Ryotaro Eda, Hisakazu Yano, Ryuichi Nakano, Yoko Takayama, Shotaro Maehana, Hidero Kitasato, Masaki Nakamura
Rok vydání: 2020
Předmět:
DNA
Bacterial
0301 basic medicine
Microbiology (medical)
Gene Transfer
Horizontal
Klebsiella pneumoniae
viruses
030106 microbiology
Microbial Sensitivity Tests
Biology
Ceftazidime
Polymerase Chain Reaction
beta-Lactam Resistance
beta-Lactamases
Microbiology
03 medical and health sciences
Minimum inhibitory concentration
0302 clinical medicine
Plasmid
Bacterial Proteins
Japan
Multiplex polymerase chain reaction
Prevalence
Humans
Pharmacology (medical)
030212 general & internal medicine
Typing
Enterobacteriaceae Infections
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Enterobacteriaceae
Hospitals
Anti-Bacterial Agents
Citrobacter freundii
Imipenem
Carbapenem-Resistant Enterobacteriaceae
Infectious Diseases
Genes
Bacterial
Multilocus sequence typing
Multilocus Sequence Typing
Plasmids
Zdroj: Journal of Infection and Chemotherapy. 26:667-671
ISSN: 1341-321X
DOI: 10.1016/j.jiac.2020.02.002
Popis: Background The increasing number of carbapenemase-producing Enterobacteriaceae (CPE) has become a global problem. Most carbapenemases detected in Japan are imipenemase, which is an imipenem-degrading enzyme with low ability; thus, CPE could have been overlooked. Therefore, this study aimed to detect and analyze CPE, without overlooking CPE showing the low minimum inhibitory concentration phenotype. Methods CPE screening was conducted on 531 ceftazidime-resistant Enterobacteriaceae isolated from Kitasato University Hospital during 2006–2015. We confirmed the presence of the carbapenemase genes (blaIMP, blaVIM, blaKPC, blaNDM, and blaOXA-48) by multiplex polymerase chain reaction. The detected CPE strains were analyzed by antimicrobial susceptibility testing, multilocus sequence typing, conjugal experiments, replicon typing, and plasmid profiling by restriction enzyme treatment. Results The CPE detection rate in Kitasato University Hospital within the past 10 years was 0.0003% (nine CPE strains). These nine CPE strains were identified to harbor 8 blaIMP-1 or 1 blaNDM-5. The CPE strains consisted of five species including Klebsiella pneumoniae and Citrobacter freundii. Six of eight blaIMP-1 were coded by IncHI2 plasmid, and the other two were coded by IncA/C plasmid. Plasmid profiling revealed that K. pneumoniae and C. freundii isolated from the same patient harbored the same plasmid. Conclusion The CPE detection rate in this study was significantly lower than those previously reported in Japan. In one case, IncA/C plasmid transmission through different bacterial species within the body was speculated. Although the number of CPE detected was low, these results indicated that the resistance plasmid could spread to other bacterial species.
Databáze: OpenAIRE
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