The arbuscular mycorrhizal fungi colonising roots and root nodules of New Zealand kauri Agathis australis
| Autor: | Mahajabeen Padamsee, Stephen E. Williams, S. Alexander Stuckey, Renee Johansen, John E. Hooker, Stanley E. Bellgard, Bruce R. Burns |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Root nodule Bioinformatics Araucariaceae Plant Science Biology 01 natural sciences DNA Ribosomal Plant Roots Glomeromycota 03 medical and health sciences Symbiosis Mycorrhizae Botany Genetics RNA Ribosomal 18S Cluster Analysis DNA Fungal Agathis australis Ecology Evolution Behavior and Systematics Phylogeny Podocarpaceae Microscopy Ecology Phytophthora agathidicida fungi Edaphic Genes rRNA RNA Fungal Sequence Analysis DNA biology.organism_classification Phylogenetics Colonisation Tracheophyta 030104 developmental biology Infectious Diseases Ultrastructure Metagenomics Root Nodules Plant 010606 plant biology & botany New Zealand |
| Zdroj: | Fungal biology. 120(5) |
| ISSN: | 1878-6146 |
| Popis: | As the only endemic member in New Zealand of the ancient conifer family, Araucariaceae, Agathis australis is an ideal species to study putatively long-evolved mycorrhizal symbioses. However, little is known about A. australis root and nodular arbuscular mycorrhizal fungi (AMF), and how mycorrhizal colonisation occurs. We used light, scanning and transmission electron microscopy to characterise colonisation, and 454-sequencing to identify the AMF associated with A. australis roots and nodules. We interpreted the results in terms of the edaphic characteristics of the A. australis-influenced ecosystem. Representatives of five families of Glomeromycota were identified via high-throughput pyrosequencing. Imaging studies showed that there is abundant, but not ubiquitous, colonisation of nodules, which suggests that nodules are mostly colonised by horizontal transmission. Roots were also found to harbour AMF. This study is the first to demonstrate the multiple Glomeromycota lineages associated with A. australis including some that may not have been previously detected. |
| Databáze: | OpenAIRE |
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