Depletion interaction forces contribute to erythrocyte-endothelial adhesion in diabetes
| Autor: | Björn Neu, Rinkoo Dalan, Melvin Khee-Shing Leow, Herbert J. Meiselman, Rani Kaliyaperumal, Xiaopeng Deng, Hao Song |
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| Přispěvatelé: | Lee Kong Chian School of Medicine (LKCMedicine), School of Chemical and Biomedical Engineering, Tan Tock Seng Hospital, Duke-NUS Medical School |
| Rok vydání: | 2019 |
| Předmět: |
Adult
0301 basic medicine Erythrocytes Endothelium Biophysics Cell Communication Type 2 diabetes Biochemistry 03 medical and health sciences 0302 clinical medicine Nonreactive Polymers Diabetes mellitus Cell Adhesion Human Umbilical Vein Endothelial Cells medicine Humans Medicine [Science] Cell adhesion Molecular Biology Chemistry Endothelial Cells Cell Biology Adhesion Middle Aged medicine.disease Blood proteins Cell biology Red blood cell 030104 developmental biology medicine.anatomical_structure Diabetes Mellitus Type 2 Red Blood Cell 030220 oncology & carcinogenesis Alternative complement pathway |
| Zdroj: | Biochemical and Biophysical Research Communications. 516:144-148 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2019.06.018 |
| Popis: | Abnormal adhesion of red blood cells (RBC) to the endothelium has been linked to the pathophysiology of several diseases associated with vascular disorders. Various biochemical changes on the outer membrane of RBC, as well as plasma protein levels, have been identified as possibly playing key roles, but the detailed interplay between plasma factors and cellular factors often remains unclear. In this work, we identified an alternative pathway by demonstrating that non-adsorbing macromolecules can also have a marked impact on the adhesion efficiency of RBC from patients with type 2 Diabetes (T2DM) to endothelial cells (EC). RBC isolated from blood samples of T2DM patients were suspended in isotonic solutions of dextran in order to mimic the impact of non-adsorbing macromolecules. Static and continuous flow adhesion assays were used to determine the adhesion behavior of T2DM RBC with EC and the results compared with those of normal controls. We found that the presence of non-adsorbing molecules promotes an increase in T2DM RBC - EC adhesion and that these RBC exhibit much greater adhesion than normal red cells. Our results thus suggest that the depletion mechanism might be an alternative phenomenon through which plasma proteins could cause enhanced RBC-EC adhesion in diabetes mellitus. These findings contribute towards the comprehensive understanding of pathophysiological mechanisms of vascular complications in diabetes and other diseases with similar vascular sequelae. Agency for Science, Technology and Research (A*STAR) Ministry of Education (MOE) National Research Foundation (NRF) This work was supported by grants from the Ministry of Education (Singapore), the National Research Foundation (Singapore) and from the Agency for Science, Technology and Research (A*Star, Singapore, BMRC grant 05/1/22/19/382). |
| Databáze: | OpenAIRE |
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