Non-functionalized soft alginate hydrogel promotes locomotor recovery after spinal cord injury in a rat hemimyelonectomy model
| Autor: | Roberta Galli, Robert Later, Ortrud Uckermann, Kerim Hakan Sitoci-Ficici, Gabriele Schackert, Matthias Kirsch, Elke Leipnitz, Michael Gelinsky, Chrysanthy Ikonomidou, Marina Matyash |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Alginates Motor Activity Matrix (biology) medicine.disease_cause Open field Rats Sprague-Dawley Cicatrix 03 medical and health sciences 0302 clinical medicine medicine Animals Rats Wistar Spinal cord injury Spinal Cord Injuries Swimming business.industry Hydrogels Histology Recovery of Function Spinal cord medicine.disease Rats Disease Models Animal 030104 developmental biology medicine.anatomical_structure Spinal Cord Self-healing hydrogels Female Surgery Neurology (clinical) Implant business Locomotion 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Acta Neurochirurgica. 160:449-457 |
| ISSN: | 0942-0940 0001-6268 |
| Popis: | Spinal cord injury (SCI) and the consecutive devastating neurological sequelae have an enormous individual and economic impact. Implantation of functionalized hydrogels is a promising approach, because they can serve as a matrix for the regenerating tissue, carry and release bioactive molecules and various cell types. We already demonstrated that non-functionalized soft alginate hydrogel supported axonal outgrowth and protected neurons against oxidative stress in vitro. Here, we investigated the effects of such soft alginate hydrogels on locomotor recovery in small and large spinal cord lesions. Hemimyelonectomy of 2 mm or 4 mm length was performed in rats and soft alginate hydrogel was implanted. Functional recovery of the hindlimbs was assessed in the open field [Batto Beattie Bresnahan (BBB) score] and using swimming test [Louisville Swim score (LSS)] for 140 days post injury (DPI). Reference histology was performed. Rats that received an alginate implant into 2 mm spinal cord lesions demonstrated significantly improved locomotor recovery compared to controls detectable already at 10 DPI. At 140 DPI, they reached higher LSS and BBB scores in swimming and open field tests, respectively. However, this beneficial effect of alginate was lacking in animals with larger (4 mm) lesions. Histological examination suggested that fibrous scarring in the spinal cord was reduced after alginate implantation in comparison to controls. Implantation of soft alginate hydrogel in small spinal cord lesions improved functional recovery. Possible underlying mechanisms include the mechanical stabilization of the wound, reduction of secondary damage and inhibition of fibrous scarring. |
| Databáze: | OpenAIRE |
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