Plasma membrane calcium pump activity in rat pancreatic islets: an accurate method to measure its calcium-dependent modulation
| Autor: | M. E. Garcia, Juan José Gagliardino, M I Borelli, Jyoti Pande, María Eugenia Alzugaray, Ashok K. Grover, Juan Pablo F.C. Rossi, M. M. Echarte, A. M. Villamil |
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| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Biophysics Stimulation Biology In Vitro Techniques Biochemistry Islets of Langerhans Plasma Membrane Calcium-Transporting ATPases Internal medicine medicine Plasma Membrane Calcium Pump Animals Phosphorylation Rats Wistar Insulin secretion Insulin Pancreatic islets Cell Membrane Cell Biology General Medicine Calcium dependent Rats Isoenzymes Endocrinology medicine.anatomical_structure Glucose Plasma membrane Ca2+ ATPase Calcium Ion Channel Gating |
| Zdroj: | Cell biochemistry and biophysics. 46(3) |
| ISSN: | 1085-9195 |
| Popis: | The aim of this study was to quantify the glucose modulation of the plasma membrane calcium pump (PMCA) function in rat pancreatic islets. Ca2+-ATPase activity and levels of phosphorylated PMCA intermediates both transiently declined to a minimum in response to stimulation by glucose. Strictly dependent on Ca2+ concentration, this inhibitory effect was fully expressed at physiological concentrations of the cation (less than 0.5 muM), then progressively diminished at higher concentrations. These results, together with those previously reported on the effects of insulin secretagogues and blockers on the activity, expression and cellular distribution of the PMCA, support the concept that the PMCA plays a key role in the regulation of Ca2+ signaling and insulin secretion in pancreatic islets. |
| Databáze: | OpenAIRE |
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