First Isolates of Leptospira spp., from Rodents Captured in Angola
| Autor: | Teresa Carreira, Elsa Fortes-Gabriel, Maria Luisa Vieira, Elsa Gabriel |
|---|---|
| Přispěvatelé: | Instituto de Higiene e Medicina Tropical (IHMT), Vector borne diseases and pathogens (VBD), Global Health and Tropical Medicine (GHTM) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Rodent Diseases Rodent animal diseases 030231 tropical medicine 030106 microbiology Biology Polymerase Chain Reaction Microbiology law.invention Mice 03 medical and health sciences 0302 clinical medicine SDG 3 - Good Health and Well-being law Leptospira Virology biology.animal medicine Animals Leptospirosis Typing Phylogeny Polymerase chain reaction Articles bacterial infections and mycoses medicine.disease biology.organism_classification Rats Infectious Diseases Angola Parasitology Leptospira interrogans |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene |
| ISSN: | 1476-1645 0002-9637 |
| DOI: | 10.4269/ajtmh.15-0027 |
| Popis: | Rodents play an important role in the transmission of pathogenic Leptospira spp. However, in Angola, neither the natural reservoirs of these spirochetes nor leptospirosis diagnosis has been considered. Regarding this gap, we captured rodents in Luanda and Huambo provinces to identify circulating Leptospira spp. Rodent kidney tissue was cultured and DNA amplified and sequenced. Culture isolates were evaluated for pathogenic status and typing with rabbit antisera; polymerase chain reaction (PCR) and sequencing were also performed. A total of 37 rodents were captured: Rattus rattus (15, 40.5%), Rattus norvegicus (9, 24.3%), and Mus musculus (13, 35.2%). Leptospiral DNA was amplified in eight (21.6%) kidney samples. From the cultures, we obtained four (10.8%) Leptospira isolates belonging to the Icterohaemorrhagiae and Ballum serogroups of Leptospira interrogans and Leptospira borgpetersenii genospecies, respectively. This study provides information about circulating leptospires spread by rats and mice in Angola. publishersversion published |
| Databáze: | OpenAIRE |
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