CHEMICAL COMPOSITION, SEROLOGICAL REACTIVITY AND ENDOTOXICITY OF LIPOPOLYSACCHARIDES EXTRACTED IN DIFFERENT WAYS FROM BACTEROIDES FRAGILIS, BACTEROIDES MELANINOGENICUS AND BACTEROIDES ORALIS
| Autor: | G. Dahlén, T. Hofstad, K. Sveen |
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| Rok vydání: | 2009 |
| Předmět: |
Lipopolysaccharides
chemistry.chemical_classification biology Polysaccharides Bacterial food and beverages General Medicine biology.organism_classification Polysaccharide Serology Microbiology Bacteroides fragilis Endotoxins chemistry.chemical_compound Biochemistry chemistry Bacteroides lipids (amino acids peptides and proteins) Reactivity (chemistry) Ultracentrifuge Trichloroacetic acid Chemical composition Prevotella melaninogenica Bacteroides melaninogenicus |
| Zdroj: | Acta Pathologica Microbiologica Scandinavica Section B Microbiology. :262-270 |
| ISSN: | 0304-131X |
| DOI: | 10.1111/j.1699-0463.1977.tb01972.x |
| Popis: | Lipopolysaccharides (LPS) extracted from strains of Bacteroides fragilis, Bacteroides melaninogenicus and Bacteroides oralis with phenol-water, trichloroacetic acid, EDTA or liquid phenol-chloroform-petroleum ether (PCP) and isolated by ultracentrifugation, varied considerably in their quantitative chemical composition. Negligible yields of LPS were obtained by PCP-extraction. All preparations were more or less serologically active. All methods (except PCP) extracted the same O-antigenic determinants from B. fragilis. Endotoxic activity, as measured by primary skin inflammations in rabbits, was low but was present in all preparations. Proteins (and/or lipoproteins) co-precipitated with LPS in the ultracentrifuge. |
| Databáze: | OpenAIRE |
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