Capture Hi-C identifies the chromatin interactome of colorectal cancer risk loci
| Autor: | Jäger, Roland, Migliorini, Gabriele, Henrion, Marc, Kandaswamy, Radhika, Speedy, Helen E, Heindl, Andreas, Whiffin, Nicola, Carnicer, Maria J, Broome, Laura, Dryden, Nicola, Nagano, Takashi, Schoenfelder, Stefan, Enge, Martin, Yuan, Yinyin, Taipale, Jussi, Fraser, Peter, Fletcher, Olivia, Houlston, Richard S |
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| Přispěvatelé: | Schoenfelder, Stefan [0000-0002-3200-8133], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Ja¨ger, R, Migliorini, G, Henrion, M, Kandaswamy, R, E. Speedy, H, Heindl, A, Whiffin, N, J. Carnicer, M, Broome, L, Dryden, N H, Nagano, T, Schoenfelder, S, Enge, M, Yuan, Y, Taipale, J, Fraser, P, Fletcher, O & Houlston, R S 2015, ' Capture Hi-C identifies the chromatin interactome of colorectal cancer risk loci ', Nature Communications, vol. 6, 6178 . https://doi.org/10.1038/ncomms7178 |
| Popis: | Multiple regulatory elements distant from their targets on the linear genome can influence the expression of a single gene through chromatin looping. Chromosome conformation capture implemented in Hi-C allows for genome-wide agnostic characterization of chromatin contacts. However, detection of functional enhancer-promoter interactions is precluded by its effective resolution that is determined by both restriction fragmentation and sensitivity of the experiment. Here we develop a capture Hi-C (cHi-C) approach to allow an agnostic characterization of these physical interactions on a genome-wide scale. Single-nucleotide polymorphisms associated with complex diseases often reside within regulatory elements and exert effects through long-range regulation of gene expression. Applying this cHi-C approach to 14 colorectal cancer risk loci allows us to identify key long-range chromatin interactions in cis and trans involving these loci. |
| Databáze: | OpenAIRE |
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