Low Force Decelerates L-selectin Dissociation from P-selectin Glycoprotein Ligand-1 and Endoglycan
| Autor: | Cheng Zhu, Tadayuki Yago, Arkadiusz G. Klopocki, Claudia B. Fieger, Krishna K. Sarangapani, Steven D. Rosen, Rodger P. McEver, Michael B. Lawrence |
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| Rok vydání: | 2004 |
| Předmět: |
Membrane Glycoproteins
biology Ligand Chemistry Mucins Catch bond CHO Cells Cell Biology Slip (materials science) Microscopy Atomic Force Biochemistry Dissociation (chemistry) ENDOGLYCAN Crystallography Cricetinae biology.protein Shear stress Biophysics Animals Humans P-selectin glycoprotein ligand-1 L-selectin L-Selectin Molecular Biology |
| Zdroj: | Journal of Biological Chemistry. 279:2291-2298 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m310396200 |
| Popis: | Selectin-ligand interactions mediate the tethering and rolling of circulating leukocytes on vascular surfaces during inflammation and immune surveillance. To support rolling, these interactions are thought to have rapid off-rates that increase slowly as wall shear stress increases. However, the increase of off-rate with force, an intuitive characteristic named slip bonds, is at odds with a shear threshold requirement for selectin-mediated cell rolling. As shear drops below the threshold, fewer cells roll and those that do roll less stably and with higher velocity. We recently demonstrated a low force regime where the off-rate of P-selectin interacting with P-selectin glycoprotein ligand-1 (PSGL-1) decreased with increasing force. This counter-intuitive characteristic, named catch bonds, might partially explain the shear threshold phenomenon. Because L-selectin-mediated cell rolling exhibits a much more pronounced shear threshold, we used atomic force microscopy and flow chamber experiments to determine off-rates of L-selectin interacting with their physiological ligands and with an antibody. Catch bonds were observed at low forces for L-selectin-PSGL-1 interactions coinciding with the shear threshold range, whereas slip bonds were observed at higher forces. These catch-slip transitional bonds were also observed for L-selectin interacting with endoglycan, a newly identified PSGL-1-like ligand. By contrast, only slip bonds were observed for L-selectin-antibody interactions. These findings suggest that catch bonds contribute to the shear threshold for rolling and are a common characteristic of selectin-ligand interactions. |
| Databáze: | OpenAIRE |
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