Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations

Autor:	Teal M. Brechtel, Ritesh Tandon, Molly Tyner
Rok vydání:	2013
Předmět:	Whole genome sequencing Genetics Bacterial artificial chromosome Genetically engineered Mutant Congenital cytomegalovirus infection Biology medicine.disease Virology Genome Viruses medicine Molecular Biology Gene Reference genome
Zdroj:	Genome Announcements
ISSN:	2169-8287
Popis:	Here, we present the complete genome sequence of a cytomegalovirus Towne-BAC (bacterial artificial chromosome) isolate that we first genetically engineered to mutate the UL96 gene and then serially passaged in human fibroblasts to allow for the accumulation of compensatory mutations. A total of 17 single-base substitutions were discovered in the passaged genome compared to the reference sequence (KF493877).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8743b4582134bd1a78cad1dd3133a4df https://doi.org/10.1128/genomea.00901-13 Zobrazit plný text záznamu