The cannabinoid receptor cb1 stabilizes sperm chromatin condensation status during epididymal transit by promoting disulphide bond formation
| Autor: | Gilda Cobellis, Lucia Altucci, Riccardo Pierantoni, Teresa Chioccarelli, Francesco Manfrevola, Veronica Porreca, Silvia Fasano |
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| Přispěvatelé: | Chioccarelli, T., Manfrevola, F., Porreca, V., Fasano, S., Altucci, L., Pierantoni, R., Cobellis, G. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Protamine thiol oxidation Sperm chromatin condensation and maturation Chromatin remodeling and histone displacement Chromodomain lcsh:Chemistry Histones Gene Knockout Techniques Mice 0302 clinical medicine Spermiogenesi Receptor Cannabinoid CB1 Disulfides lcsh:QH301-705.5 Spectroscopy Epididymis biology Sperm chromatin condensation Chemistry Nuclear Proteins caput and cauda epididymis Acetylation General Medicine Spermatozoa Chromatin Computer Science Applications Histone displacement Cell biology Histone 030220 oncology & carcinogenesis lipids (amino acids peptides and proteins) Co-Repressor Proteins endocrine system Chromodomain protein CDYL Article Catalysis Chromatin remodeling Estrogens and endocannabinoid Inorganic Chemistry Histone H4 03 medical and health sciences Prophase estrogens and endocannabinoids Animals Physical and Theoretical Chemistry Molecular Biology Hydro-Lyases urogenital system Bromodomain protein BRDT Organic Chemistry Chromatin Assembly and Disassembly spermiogenesis Type-1 cannabinoid receptor (CB1) 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Gene Expression Regulation biology.protein Post-translational modification of histone H4 (histone H4 acetylation) Caput and cauda epididymi Gene Deletion |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 21, Iss 3117, p 3117 (2020) Volume 21 Issue 9 |
| Popis: | The cannabinoid receptor CB1 regulates differentiation of spermatids. We recently characterized spermatozoa from caput epididymis of CB1-knock-out mice and identified a considerable number of sperm cells with chromatin abnormality such as elevated histone content and poorly condensed chromatin. In this paper, we extended our findings and studied the role of CB1 in the epididymal phase of chromatin condensation of spermatozoa by analysis of spermatozoa from caput and cauda epididymis of wild-type and CB1-knock-out mouse in both a homozygous or heterozygous condition. Furthermore, we studied the impact of CB1-gene deletion on histone displacement mechanism by taking into account the hyperacetylation of histone H4 and players of displacement such as Chromodomain Y Like protein (CDYL) and Bromodomain testis-specific protein (BRDT). Our results show that CB1, via local and/or endocrine cell-to-cell signaling, modulates chromatin remodeling mechanisms that orchestrate a nuclear condensation extent of mature spermatozoa. We show that CB1-gene deletion affects the epididymal phase of chromatin condensation by interfering with inter-/intra-protamine disulphide bridges formation, and deranges the efficiency of histone removal by reducing the hyper-acetylation of histone H4. This effect is independent by gene expression of Cdyl and Brdt mRNA. Our results reveal a novel and important role for CB1 in sperm chromatin condensation mechanisms. |
| Databáze: | OpenAIRE |
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