Molecular cloning, characterization and functional analysis of GluCl from the oriental armyworm, Mythimna separata Walker
| Autor: | Ran Wang, San-Ji Gao, Ji-han Zhao, Li-fei Chen, Rong Wang, Da Xiao, Jinda Wang, Dong-jiang Lin, Jia-song Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Insecticides DNA Complementary Health Toxicology and Mutagenesis media_common.quotation_subject Insect Moths Biology Molecular cloning 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Mythimna separata Chloride Channels RNA interference Complementary DNA Animals RNA Messenger Cloning Molecular Gene media_common Genetics Ivermectin Alternative splicing General Medicine biology.organism_classification Alternative Splicing 010602 entomology 030104 developmental biology chemistry Abamectin Agronomy and Crop Science |
| Zdroj: | Pesticide Biochemistry and Physiology. 156:56-62 |
| ISSN: | 0048-3575 |
| Popis: | Glutamate-gated chloride channels (GluCls) mediate inhibitory synaptic transmission in invertebrate nervous systems, and only one GluCl gene has been found in insects. Therefore, insect GluCls are one of the major targets of insecticides including avermectins. In the present study, a 1347 bp full-length cDNA encoding a 449-amino acid protein (named MsGluCl, GenBank ID: MK336885) was cloned from the oriental armyworm, Mythimna separata, and characterized two alternative splicing variants of MsGluCl. The protein shares 76.9–98.6% identity with other insect GluCl isoforms. Spatial and temporal expression analysis revealed that MsGluCl was highly expressed in the 3rd instar and adult head. Dietary ingestion of dsMsGluCl significantly reduced the mRNA level of MsGluCl and decreased abamectin mortality. Thus, our results reveal that MsGluCl could be the molecular target of abamectin and provide the basis for further understanding the resistance mechanism to abamectin in arthropods. |
| Databáze: | OpenAIRE |
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