Inhibition of polyamine oxidase activity affects tumor development during the maize-Ustilago maydis interaction
| Autor: | Margarita Juárez-Montiel, Margarita Rodríguez-Kessler, María Elisa Gonzalez, Alicia Becerra-Flora, Fernando Luis Pieckenstain, Juan Francisco Jiménez-Bremont, Francisco Ignacio Jasso-Robles, Ramón Fernando García-de la Cruz |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Ustilago Otras Ciencias Biológicas Plant Tumors 1 8-DIAMINOOCTANE CELL ELONGATION POLYAMINE OXIDASE Plant Science Biology Zea mays 01 natural sciences Microbiology Ciencias Biológicas 03 medical and health sciences chemistry.chemical_compound USTILAGO MAYDIS Genetics Extracellular BIOTIC STRESS HYDROGEN PEROXIDE Secondary metabolism Plant Proteins Oxidoreductases Acting on CH-NH Group Donors Catabolism food and beverages Biotic stress PLANT TUMORS biology.organism_classification 030104 developmental biology chemistry Biochemistry Host-Pathogen Interactions Polyamine Polyamine oxidase CIENCIAS NATURALES Y EXACTAS 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry. 102:115-124 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2016.02.019 |
| Popis: | Ustilago maydis is a biotrophic plant pathogenic fungus that leads to tumor development in the aerial tissues of its host, Zea mays. These tumors are the result of cell hypertrophy and hyperplasia, and are accompanied by the reprograming of primary and secondary metabolism of infected plants. Up to now, little is known regarding key plant actors and their role in tumor development during the interaction with U. maydis. Polyamines are small aliphatic amines that regulate plant growth, development and stress responses. In a previous study, we found substantial increases of polyamine levels in tumors. In the present work, we describe the maize polyamine oxidase (PAO) gene family, its contribution to hydrogen peroxide (H2O2) production and its possible role in tumor development induced by U. maydis. Histochemical analysis revealed that chlorotic lesions and maize tumors induced by U. maydis accumulate H2O2 to significant levels. Maize plants inoculated with U. maydis and treated with the PAO inhibitor 1,8-diaminooctane exhibit a notable reduction of H2O2 accumulation in infected tissues and a significant drop in PAO activity. This treatment also reduced disease symptoms in infected plants. Finally, among six maize PAO genes only the ZmPAO1, which encodes an extracellular enzyme, is up-regulated in tumors. Our data suggest that H2O2 produced through PA catabolism by ZmPAO1 plays an important role in tumor development during the maize-U. maydis interaction. Fil: Jasso Robles, Francisco Ignacio. Universidad Autónoma de San Luis Potosí. Facultad de Ciencias; México Fil: Jiménez Bremont, Juan Francisco. Instituto Potosino de Investigación Científica y Tecnológica; México Fil: Becerra Flora, Alicia. Instituto Potosino de Investigación Científica y Tecnológica; México Fil: Juárez Montiel, Margarita. Instituto Potosino de Investigación Científica y Tecnológica; México Fil: Gonzalez, Maria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Pieckenstain, Fernando Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: García de la Cruz, Ramón Fernando. Universidad Autónoma de San Luis Potosí. Facultad de Ciencias Químicas; México Fil: Rodríguez Kessler, Margarita. Universidad Autónoma de San Luis Potosí. Facultad de Ciencias; México |
| Databáze: | OpenAIRE |
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