Coamplification of 12p11 and 12q13∼q22 in multiple ring chromosomes in a spindle cell sarcoma resolved by novel multicolor fluorescence in situ hybridization analysis
| Autor: | Valia S. Lestou, Olga Ludkovski, Helena Gosling, John X. O'Connell, Douglas E. Horsman, David Lesack |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Genetic Markers
Male Cancer Research Marker chromosome Ring chromosome Biology Chromosome 18 Complex Karyotype Genetics medicine Humans Ring Chromosomes Molecular Biology In Situ Hybridization Fluorescence Chromosome 12 Anaphase Chromosomes Human Pair 12 Chromosomes Human Pair 13 medicine.diagnostic_test Gene Amplification Chromosome Mapping Sarcoma Middle Aged medicine.disease Molecular biology Karyotyping Spindle cell sarcoma Chromosomes Human Pair 9 Fluorescence in situ hybridization |
| Zdroj: | Cancer Genetics and Cytogenetics. 139:44-47 |
| ISSN: | 0165-4608 |
| Popis: | An 80-year-old male presented with a lobulated mass in the lower abdominal wall. A diagnosis of an intermediate grade myofibroblastic spindle cell sarcoma was made. Cytogenetic analysis demonstrated a complex karyotype with a der(6), a small marker and five, different in size, ring chromosomes. Fluorescence in situ hybridization (FISH), multiplex FISH, and multicolor banding analysis was used to further delineate this complex karyotype. The der(6) was shown to be a der(18)t(6;18;9;12;18), the marker chromosome was identified as del(17), and the ring chromosomes as r(9) and r(12;18)×4. Amplification of 18 and coamplification of 12p and 12q was detected in the ring and marker chromosomes. No intercellular heterogeneity was observed although a few micronuclei containing chromosome 18 and anaphase bridges, containing chromosome 12 material, the result of bridge–fusion–bridge (BFB) cycles, were observed. Our findings combined with results from others indicate that amplification of chromosomes 12 and 18 as well as BFB phenomena characterize this type of sarcoma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect