Meningeal lymphatic endothelial cells fulfill scavenger endothelial cell function and cooperate with microglia in waste removal from the brain
Autor: | Stefan Schulte-Merker, Ulrich Dobrindt, Andreas van Impel, Thomas Zobel, Katharina Uphoff, Mario Schelhaas, Michael F. Berger, Yvonne Huisman, Jeroen Bussmann |
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Rok vydání: | 2021 |
Předmět: |
Cell type
scavenger endothelial cells government.form_of_government Population microglia Biology Cellular and Molecular Neuroscience Meninges meningeal lymphatic endothelial cells Parenchyma medicine Extracellular Animals education Zebrafish education.field_of_study Microglia macromolecular uptake Brain Endothelial Cells zebrafish biology.organism_classification Cell biology Endothelial stem cell Lymphatic Endothelium medicine.anatomical_structure brain lymphatic endothelial cells Neurology government |
Zdroj: | GLIA, 70(1), 35-49. WILEY Glia, 70(1), 35-49. WILEY |
ISSN: | 1098-1136 0894-1491 |
DOI: | 10.1002/glia.24081 |
Popis: | Brain lymphatic endothelial cells (BLECs) constitute a group of loosely connected endothelial cells that reside within the meningeal layer of the zebrafish brain without forming a vascular tubular system. BLECs have been shown to readily endocytose extracellular cargo molecules from the brain parenchyma, however, their functional relevance in relation to microglia remains enigmatic. We here compare their functional uptake efficiency for several macromolecules and bacterial components with microglia in a qualitative and quantitative manner in 5-day-old zebrafish embryos. We find BLECs to be significantly more effective in the uptake of proteins, polysaccharides and virus particles as compared to microglia, while larger particles like bacteria are only ingested by microglia but not by BLECs, implying a clear distribution of tasks between the two cell types in the brain area. In addition, we compare BLECs to the recently discovered scavenger endothelial cells (SECs) of the cardinal vein and find them to accept an identical set of substrate molecules. Our data identifies BLECs as the first brain-associated SEC population in vertebrates, and demonstrates that BLECs cooperate with microglia to remove particle waste from the brain. |
Databáze: | OpenAIRE |
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