Prevelance of Common YMDD Motif Mutations in Long Term Treated Chronic HBV Infections in a Turkish Population
| Autor: | Abdulkerim Yilmaz, Mahmut Coskun, Hakan Alagozlu, Binnur Koksal, Öztürk Özdemir |
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| Přispěvatelé: | [Alagozlu, Hakan -- Yilmaz, Abdulkerim] Cumhuriyet Univ, Fac Med, Dept Gastroenterol, Sivas, Turkey -- [Koksal, Binnur] Cumhuriyet Univ, Fac Med, Dept Med Genet, Sivas, Turkey -- [Ozdemir, Ozturk] Canakkale Onsekiz Mart Univ, Fac Med, Dept Med Genet, Canakkel, Turkey -- [Ozdemir, Ozturk -- Coskun, Mahmut] Canakkale Onsekiz Mart Univ, Fac Med, Dept Med Biol, Canakkel, Turkey, Coskun, Mahmut -- 0000-0002-6174-3129 |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Cancer Research polymerase gene mutation Turkey Epidemiology HCC risk Amino Acid Motifs Drug resistance DNA-Directed DNA Polymerase long term treatment Methionine Adefovir HBV Missense mutation Transversion education.field_of_study virus diseases Lamivudine Hepatitis B Middle Aged Prognosis Oncology Female medicine.drug Adult Hepatitis B virus Anti-HIV Agents Population Organophosphonates Biology Antiviral Agents Hepatitis B Chronic Drug Resistance Viral medicine Humans education Aged Retrospective Studies Aspartic Acid Point mutation Adenine YMDD Public Health Environmental and Occupational Health medicine.disease Virology Molecular biology digestive system diseases Case-Control Studies DNA Viral Mutation Tyrosine Follow-Up Studies |
| Popis: | WOS: 000328273000092 PubMed ID: 24175847 In the current study we aimed to show the common YMDD motif mutations in viral polymerase gene in chronic hepatitis B patients during lamivudine and adefovir therapy. Forty-one serum samples obtained from chronic hepatitis B patients (24 male, 17 female; age range: 34-68 years) were included in the study. HBV-DNA was extracted from the peripheral blood of the patients using an extraction kit (Invisorb, Instant Spin DNA/RNA Virus Mini Kit, Germany). A line probe assay and direct sequencing analyses (INNO-LIPA HBV DR v2; INNOGENETICS N.V, Ghent, Belgium) were applied to determine target mutations of the viral polymerase gene in positive HBV-DNA samples. A total of 41 mutations located in 21 different codons were detected in the current results. In 17 (41.5%) patients various point mutations were detected leading to lamivudin, adefovir and/or combined drug resistance. Wild polymerase gene profiles were detected in 24 (58.5%) HBV positive patients of the current cohort. Eight of the 17 samples (19.5%) having rtM204V/I/A missense transition and/or transversion point mutations and resistance to lamivudin. Six of the the mutated samples (14.6%) having rtL180M missense transversion mutation and resistance to combined adefovir and lamivudin. Three of the mutated samples (7.5%) having rtG215H by the double base substituation and resistance to adefovir. Three of the mutated samples (7.5%) having codon rtL181W due to the missense transversion point mutations and showed resistance to combined adefovir and lamivudin. Unreported novel point mutations were detected in the different codons of polymerase gene region in the current HBV positive cohort from Turkish population. The current results provide evidence that rtL180M and rtM204V/I/A mutations of HBV-DNA may be associated with a poor antiviral response and HBV chronicity during conventional therapy in Turkish patients. |
| Databáze: | OpenAIRE |
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