Time-resolved polarization measurements of porphyrin fluorescence in solution and in single cells
| Autor: | H.K. Seidlitz, K. Stettmaier, Herbert Schneckenburger |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Radiation
Porphyrins Time Factors Radiological and Ultrasound Technology Chemistry Biophysics Fluorescence spectrometry Protoporphyrins Fluorescence Polarization Photochemistry Fluorescence Porphyrin Epithelium Cell Line Rats Solutions chemistry.chemical_compound Resonance fluorescence Fluorescence microscope Animals Radiology Nuclear Medicine and imaging Fluorescence cross-correlation spectroscopy Laser-induced fluorescence Vero Cells Fluorescence anisotropy |
| Zdroj: | Journal of photochemistry and photobiology. B, Biology. 5(3-4) |
| ISSN: | 1011-1344 |
| Popis: | Porphyrin monomers, dimers and aggregates, which can be differentiated on the basis of their fluorescence lifetimes, are shown to possess different degrees of fluorescence polarization. This opens up new possibilities for microscopic imaging of these individual components in photosensitization and tumour detection. A rough estimate of the size of the porphyrin aggregates is obtained from the data of time-resolved fluorescence anisotropy. |
| Databáze: | OpenAIRE |
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