Multiple subcellular localizations of PCTAIRE-1 in brain
| Autor: | Françoise Le Bouffant, Elisabeth Traiffort, Fritz Sladeczek, Martial Ruat, Pascale Le Minter |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Cerebellum
Insecta Transcription Genetic Nucleolus Blotting Western Hippocampus Mice Inbred Strains Biology Protein Serine-Threonine Kinases Gene Expression Regulation Enzymologic Rats Sprague-Dawley Cellular and Molecular Neuroscience Immunolabeling Mice Open Reading Frames Purkinje Cells Antibody Specificity Neurofilament Proteins Glial Fibrillary Acidic Protein medicine Animals Mossy fiber (cerebellum) RNA Messenger Molecular Biology Cells Cultured In Situ Hybridization Pyramidal Cells Colocalization Riboprobe Cell Biology Granule cell Immunohistochemistry Cyclin-Dependent Kinases Cell biology Rats medicine.anatomical_structure Antisense Elements (Genetics) Mossy Fibers Hippocampal Rabbits Neuroscience Subcellular Fractions |
| Zdroj: | Molecular and cellular neurosciences. 16(4) |
| ISSN: | 1044-7431 |
| Popis: | We developed a selective antibody to a synthetic peptide corresponding to an N-terminal sequence of the PCTAIRE-1 protein. In rodent brain extracts it recognized only the protein doublet characteristic of PCTAIRE-1, and this signal is completely abolished by preincubation of the antibody with the immunopeptide. Immunolabeling experiments done with this PCTAIRE-1-specific antibody reveal that the protein is widely distributed in the rodent brain as are the mRNAs visualized using an antisense riboprobe corresponding to the entire PCTAIRE-1 open reading frame. Two types of PCTAIRE-1 protein localizations were observed: first a diffuse labeling of almost all brain regions, particularly intense in the molecular layer of the cerebellum and the mossy fiber region of the hippocampus, and second a spot-like localization in the nuclei of large neurons such as cerebellar Purkinje cells and pyramidal cells of the hippocampus. Colocalization with the B23 protein allows one to identify these compartments as nucleoli. Our results suggest a nucleolar function of PCTAIRE-1 in large neurons and a role in regions containing important granule cell projections. |
| Databáze: | OpenAIRE |
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