Map-based cloning of zb7 encoding an IPP and DMAPP synthase in the MEP pathway of maize
| Autor: | Weibin Song, Jinsheng Lai, Wei Chen, Mei Zhang, X. Hu, Zongliang Chen, Yongbin Dong, Yuanzeng Zhao, Zhen-Hua Wang, Xiaomin Lu |
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| Rok vydání: | 2012 |
| Předmět: |
Chloroplasts
Indoles Transgene Mutant Molecular Sequence Data Plant Science Biology medicine.disease_cause Zea mays Hemiterpenes Organophosphorus Compounds RNA interference medicine Amino Acid Sequence Cloning Molecular Molecular Biology Plant Proteins Cloning Genetics Mutation ATP synthase Chromosome Mapping Phenotype Cell biology Biosynthetic Pathways Chloroplast Erythritol biology.protein Sugar Phosphates Sequence Alignment |
| Zdroj: | Molecular plant. 5(5) |
| ISSN: | 1752-9867 |
| Popis: | IspH is a key enzyme in the last step of the methyl-D-erythritol-4-phosphate (MEP) pathway. Loss of function of IspH can often result in complete yellow or albino phenotype in many plants. Here, we report the characterization of a recessive mutant of maize, zebra7 (zb7), showing transverse green/yellow striped leaves in young plants. The yellow bands of the mutant have decreased levels of chlorophylls and carotenoids with delayed chloroplast development. Low temperature suppressed mutant phenotype, while alternate light/dark cycle or high temperature enlarged the yellow section. Map-based cloning demonstrated that zb7 encodes the IspH protein with a mis-sense mutation in a conserved region. Transgenic silencing of Zb7 in maize resulted in complete albino plantlets that are aborted in a few weeks, confirming that Zb7 is important in the early stages of maize chloroplast development. Zb7 is constitutively expressed and its expression subject to a 16-h light/8-h dark cycle regulation. Our results suggest that the less effective or unstable IspH in zb7 mutant, together with its diurnal expression, are mechanistically accounted for the zebra phenotype. The increased IspH mRNA in the leaves of zb7 at the late development stage may explain the restoration of mutant phenotype in mature stages. |
| Databáze: | OpenAIRE |
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