Neonatal exposure to agonists and antagonists of sex steroid receptors affects AMH and FSH plasma level and their receptors expression in the adult pig ovary
| Autor: | Maria Slomczynska, Marek Koziorowski, Katarzyna Knapczyk-Stwora, Malgorzata Grzesiak, Malgorzata Duda, Patrycja Witek |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Testosterone propionate
pig endocrine system medicine.medical_specialty medicine.drug_class Biology urologic and male genital diseases Article Flutamide 03 medical and health sciences chemistry.chemical_compound Follicle-stimulating hormone 0302 clinical medicine Internal medicine FSH polycyclic compounds medicine AMH Receptor 030304 developmental biology 0303 health sciences 030219 obstetrics & reproductive medicine General Veterinary Sex hormone receptor Antral follicle Androgen Androgen receptor Endocrinology chemistry endocrine active compounds Animal Science and Zoology ovary hormones hormone substitutes and hormone antagonists |
| Zdroj: | Animals Volume 10 Issue 1 Animals : an Open Access Journal from MDPI |
| Popis: | In this study piglets were injected with testosterone propionate (TP, an androgen), flutamide (FLU, an antiandrogen), 4-tert-octylphenol (OP, an estrogenic compound), ICI 182,780 (ICI, an antiestrogen) or corn oil (controls) between postnatal days 1 and 10 (N = 5/group). Then plasma anti-Mü llerian hormone (AMH) and follicle stimulating hormone (FSH) concentration and the expression of their receptors were examined in the adult pig ovary. TP and FLU decreased plasma AMH and FSH concentration. In preantral follicles, TP resulted in upregulation of AMHR2 and FSHR expression, but decreased AMH protein abundance. FLU upregulated AMHR2 expression, while OP increased FSHR mRNA. In small antral follicles, OP upregulated ACVR1 and BMPR1A expression, while FLU increased BMPR1A mRNA. FLU and ICI resulted in upregulation of AMHR2 expression. TP and FLU upregulated AMH expression, while it was downregulated in response to OP or ICI. Moreover, OP and ICI resulted in downregulation of FSHR expression, while FLU decreased FSHR protein abundance. In conclusion, neonatal exposure to either agonist or antagonist of androgen receptor affected AMH and FSH signalling systems in preantral follicles. In small antral follicles these systems were influenced by compounds with estrogenic, antiestrogenic, and antiandrogenic activity. Consequently, these hormonal agents may cause an accelerated recruitment of primordial follicles and affect the cycling recruitment of small antral follicles in pigs. |
| Databáze: | OpenAIRE |
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