Eradication of Candida albicans Biofilm Viability: In Vitro Combination Therapy of Cationic Carbosilane Dendrons Derived from 4-Phenylbutyric Acid with AgNO3 and EDTA
| Autor: | Rafael Gómez, Irene Heredero-Bermejo, Juan Soliveri, Tania Lozano-Cruz, José Luis Copa-Patiño, Paula Ortega, Natalia Gómez-Casanova |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) QH301-705.5 030106 microbiology synergy Human pathogen Plant Science Article biofilm carbosilane dendron Microbiology HeLa 03 medical and health sciences Candida albicans Biology (General) Cytotoxicity Pathogen Ecology Evolution Behavior and Systematics biology Chemistry EDTA Biofilm biochemical phenomena metabolism and nutrition biology.organism_classification In vitro Corpus albicans 030104 developmental biology silver nitrate |
| Zdroj: | Journal of Fungi Volume 7 Issue 7 Journal of Fungi, Vol 7, Iss 574, p 574 (2021) |
| ISSN: | 2309-608X |
| DOI: | 10.3390/jof7070574 |
| Popis: | Candida albicans is a human pathogen of significant clinical relevance. This pathogen is resistant to different drugs, and most clinical antifungals are not effective against the prevention and treatment of C. albicans infections. As with other microorganisms, it can produce biofilms that serve as a barrier against antifungal agents and other substances, contributing to infection in humans and environmental tolerance of this microorganism. Thus, resistances and biofilm formation make treatment difficult. In addition, the complete eradication of biofilms in implants, catheters and other medical devices, is challenging and necessary to prevent relapses of candidemia. Therefore, it is a priority to find new molecules or combinations of compounds with anti-Candida biofilm activity. Due to the difficulty of treating and removing biofilms, the aim of this study was to evaluate the in vitro ability of different generation of cationic carbosilane dendrons derived from 4-phenylbutyric acid, ArCO2Gn(SNMe3I)m, to eradicate C. albicans biofilms. Here, we assessed the antifungal activity of the second generation dendron ArCO2G2(SNMe3I)4 against C. albicans cells and established biofilms since it managed to seriously damage the membrane. In addition, the combinations of the second generation dendron with AgNO3 or EDTA eradicated the viability of biofilm cells. Alterations were observed by scanning electron microscopy and cytotoxicity was assessed on HeLa cells. Our data suggest that the dendritic compound ArCO2G2(SNMe3I)4 could represent an alternative to control the infections caused by this pathogen. |
| Databáze: | OpenAIRE |
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