Telomerase Repeat Amplification Protocol (TRAP): A New Molecular Marker for Parathyroid Carcinoma
| Autor: | Susanna Benvenuti, Valentina Martineti, L. Becherini, Lucia Picariello, Annamaria Morelli, Alberto Falchetti, Luigi Gennari, Cesare Bordi, Maria Luisa Brandi, Rinaldo Lampugnani |
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| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Telomerase DNA Mutational Analysis Biophysics Biology Malignancy Biochemistry Parathyroid Glands Internal medicine Biomarkers Tumor Tumor Cells Cultured medicine Humans Molecular Biology Cells Cultured Parathyroid neoplasm Exons Cell Biology Parathyroid chief cell medicine.disease Parathyroid Neoplasms Endocrinology Parathyroid carcinoma Cell culture Cancer research Stem cell Immortalised cell line |
| Zdroj: | Biochemical and Biophysical Research Communications. 265:252-255 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1999.1320 |
| Popis: | Telomerase results to be active in human germ, stem cells, several malignant cell tumors and in immortalized cell lines. In order to investigate if molecular mechanisms other than Rb gene inactivation can be helpful to diagnose malignancy of parathyroid tumors, we decided to investigate the presence of active telomerase in homogenates from different pathological parathyroid tissues (hyperplastic, adenomatous, carcinomatous, and normal) and primary cell cultures. The TRAP assay was performed to detect this activity in histologically characterized normal, hyperplastic, adenomatous, and carcinomatous human parathyroid tissues, primary cell lines, and one metastatic tissue from parathyroid carcinoma. Only malignant parathyroid glands and the metastatic tissue were TRAP positive. Our findings suggest that telomerase expression could represent an important molecular mechanism underlying the acquisition and progression of an aggressive phenotype of epithelial parathyroid cells and it may help to predict their malignant potential. The TRAP assay is easy to perform and it could become an additional tool to be included in the harmamentarium for the molecular diagnosis of parathyroid carcinoma. |
| Databáze: | OpenAIRE |
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