Concave ferguson plots of DNA fragments and convex ferguson plots of bacteriophages: Evaluation of molecular and fiber properties, using desktop computers
| Autor: | Dietmar Tietz, Andreas Chrambach |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Gel electrophoresis
Particle properties Chromatography Computer program Computers Clinical Biochemistry Fiber (computer science) Regular polygon DNA Biochemistry Plot (graphics) Analytical Chemistry Electrophoresis chemistry.chemical_compound Logistic Models chemistry Software Design Agarose Bacteriophages Biological system |
| Zdroj: | Electrophoresis. 13:286-294 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.1150130160 |
| Popis: | A desktop computer program evaluating physical properties of DNA and bacteriophages is presented. The analysis is based on data obtained from capillary and submarine-type agarose electrophoresis. Native molecular/particle properties and properties of the gel (or polymer) medium can be derived from electrophoresis at several gel concentrations. This is done conveniently by a computerized evaluation of the semi-logarithmic plot of mobility vs. gel concentration, designated the Ferguson plot. In application to most proteins, this plot is linear and computer programs exist to evaluate it. However, nonlinear Ferguson plots have assumed great importance in view of the fact that the plots are concave for DNA. Similarly, convex plots are important since they prevail in the electrophoresis of large particles in agarose. The computer program reported here is the first to (i) address concave Ferguson plots and (ii) allow for the evaluation of both cases using a desktop computer. Program ELPHOFIT version 2.0, a Macintosh application, is available upon request. |
| Databáze: | OpenAIRE |
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