Processing recommendations for using low-solids digestate as nutrient solution for poly-ß-hydroxybutyrate production with Synechocystis salina
| Autor: | Werner Fuchs, C. Daffert, Ines Fritz, Katharina Meixner, K. Markl, B. Drosg |
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| Rok vydání: | 2016 |
| Předmět: |
Cyanobacteria
Nitrogen Polyesters 020209 energy Hydroxybutyrates chemistry.chemical_element Biomass Photobioreactor Bioengineering Environmental pollution macromolecular substances 02 engineering and technology 010501 environmental sciences 01 natural sciences Applied Microbiology and Biotechnology Microbiology Nutrient 0202 electrical engineering electronic engineering information engineering 0105 earth and related environmental sciences biology Phosphorus Synechocystis technology industry and agriculture General Medicine Pulp and paper industry biology.organism_classification Solutions chemistry Digestate lipids (amino acids peptides and proteins) Biotechnology |
| Zdroj: | Journal of Biotechnology. 240:61-67 |
| ISSN: | 0168-1656 |
| Popis: | Within the last decades, environmental pollution with persistent plastics steadily increased; therefore the production of biodegradable materials like poly-s-hydroxybutyrate (PHB) is essential. Currently, PHB is produced with heterotrophic bacteria from crops. This leads to competition with food and feed production, which can be avoided by using photoautotrophic cyanobacteria, as Synechocystis salina, synthesizing PHB from CO2 at nutrient limitation. This study aims to increase the economic efficiency of PHB production with cyanobacteria by using nutrients from anaerobic digestate. First, growth and PHB production of S. salina in digestate fractions (supernatant and permeate, with/without precipitating agents) and dilutions thereof and then the scale-up (photobioreactor, 200 L working volume) were evaluated. With precipitated and centrifuged digestate diluted 1/3 the highest biomass (1.55gL-1) and PHB concentrations (95.4mgL-1), being 78% of those in mineral media, were achieved. In the photobioreactor-experiments biomass (1.63gL-1) and PHB concentrations (88.7mgL-1), being 79% and 72% of those in mineral medium, were reached, but in a cultivation time 10days longer than in mineral medium. The possibility to use digestate as sustainable and low cost nutrient solution for microalgae cultivation and photoautotrophic PHB production, instead of applying it on fields or processing it to achieve discharge limits, makes this application a highly valid option. |
| Databáze: | OpenAIRE |
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