Formation of α-1,2- and α-1,3-linked mannose disaccharides from dolichyl mannosyl phosphate by rat liver membrane enzymes
| Autor: | George Wolf, Helen Quill |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Guanosine Diphosphate Mannose
Male Stereochemistry Biophysics Mannose Disaccharides Mannosyltransferases Biochemistry chemistry.chemical_compound Mannosidases Animals Polyisoprenyl Phosphate Sugars Molecular Biology Incubation Chemistry Substrate (chemistry) Rats carbohydrates (lipids) Electrophoresis Paper chromatography Membrane Hexosyltransferases Liver Mannolipid Mannosides Microsome Dolichol Monophosphate Mannose |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 674:327-335 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/0304-4165(81)90363-9 |
| Popis: | Dolichyl mannosyl phosphate and GDPmannose were active substrates for the transfer of mannose to methyl-alpha-D-mannose, p-nitrophenyl-alpha-D-mannose, and free mannose with rat liver microsomal membranes. The products formed during dolichyl mannosyl phosphate incubation with methyl-alpha-D-mannose or with mannose were alpha-linked. The disaccharides formed by incubation of dolichyl mannosyl phosphate or GDPmannose with mannose were identified by paper chromatography and electrophoresis as mannose-alpha-1,2-mannose and mannose-alpha-1,3-mannose. synthesis of each product was dependent on the assay conditions used and was most markedly affected by the presence of detergent. Transfer of mannose from either substrate to form mannose-alpha-1,3-mannose was severely inhibited by Triton X-100. |
| Databáze: | OpenAIRE |
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