Interactions between Zn and Cu in LEC rats, an animal model of Wilson's disease
| Autor: | Paola Irato, Sabrina Giannetto, Valentina Medici, Alessandro Santon, Vincenzo Albergoni, Renata D'Incà, Giacomo Carlo Sturniolo |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Pathology Histology metallothionein metals immunolocalisation autofluorescence LEC rats INTESTINAL METALLOTHIONEIN COPPER-METALLOTHIONEIN ZINC ACETATE HEPATITIS IRON metals LEC rats chemistry.chemical_element Zinc Absorption (skin) autofluorescence Kidney HEPATITIS Animal model Hepatolenticular Degeneration Internal medicine INTESTINAL METALLOTHIONEIN medicine Metallothionein Animals Drug Interactions immunolocalisation Molecular Biology Rats Inbred LEC ZINC ACETATE IRON COPPER-METALLOTHIONEIN Cell Biology Metabolism medicine.disease Intestinal epithelium Immunohistochemistry Rats Wilson's disease Medical Laboratory Technology Disease Models Animal Endocrinology chemistry Liver Microscopy Fluorescence Copper |
| Zdroj: | Histochemistry and cell biology. 117(3) |
| ISSN: | 0948-6143 |
| Popis: | The effect of oral Zn treatment was studied in the liver and kidneys of 26 male Long-Evans Cinnamon (LEC) rats (mutant animals, 5 weeks old) in relation to both the interaction between Zn and Cu and the localisation and concentration of metallothionein (MT). Rats receiving 80 mg zinc acetate daily by gavage and control rats receiving no treatment were killed after 1 or 2 weeks. By immunohistochemical and analytical chemical techniques we revealed that treated rats had higher levels of MT in the hepatic and renal cells compared to untreated ones. Tissue Zn concentrations were significantly higher in treated rats compared to untreated whereas Cu concentrations decreased in the liver and kidneys as indicated by analytical chemical analyses. MT levels also decreased with treatment period. A histochemical procedure, obtained using autofluorescence of Cu-metallothioneins, confirms these findings: after 2 weeks, the signal decreased in both the liver and kidney sections. This gives a greater understanding of the mechanism of Cu metabolism in the two tissues considered. These results suggest that Zn acts both to compete for absorption on the luminal side of the intestinal epithelium and to induce the synthesis of MT. |
| Databáze: | OpenAIRE |
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