Timing of Myocardial Trpm7 Deletion During Cardiogenesis Variably Disrupts Adult Ventricular Function, Conduction, and Repolarization
| Autor: | David E. Clapham, William J. Gibson, Dipayan Chaudhuri, Marjolein A.W. van den Boogert, Matteo E. Mangoni, Christopher Bates-Withers, Pietro Mesirca, Rajan Sah, William T. Pu, Xenos Mason |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Mice 129 Strain Time Factors Heart block Action Potentials TRPM Cation Channels Biology Article TRPC1 Mice TRPC3 Heart Conduction System TRPM7 Physiology (medical) Internal medicine medicine Animals Ventricular Function Repolarization Myocytes Cardiac Mice Knockout Myocardium Age Factors medicine.disease Heart failure Knockout mouse Cardiology Electrical conduction system of the heart Cardiology and Cardiovascular Medicine Gene Deletion |
| Zdroj: | Circulation. 128:101-114 |
| ISSN: | 1524-4539 0009-7322 |
| DOI: | 10.1161/circulationaha.112.000768 |
| Popis: | Background— Transient receptor potential ( TRP ) channels are a superfamily of broadly expressed ion channels with diverse physiological roles. TRPC1, TRPC3 , and TRPC6 are believed to contribute to cardiac hypertrophy in mouse models. Human mutations in TRPM4 have been linked to progressive familial heart block. TRPM7 is a divalent-permeant channel and kinase of unknown function, recently implicated in the pathogenesis of atrial fibrillation; however, its function in ventricular myocardium remains unexplored. Methods and Results— We generated multiple cardiac-targeted knockout mice to test the hypothesis that TRPM7 is required for normal ventricular function. Early cardiac Trpm7 deletion (before embryonic day 9; TnT / Isl1-C re) results in congestive heart failure and death by embryonic day 11.5 as a result of hypoproliferation of the compact myocardium. Remarkably, Trpm7 deletion late in cardiogenesis (about embryonic day 13; αMHC-Cre ) produces viable mice with normal adult ventricular size, function, and myocardial transcriptional profile. Trpm7 deletion at an intermediate time point results in 50% of mice developing cardiomyopathy associated with heart block, impaired repolarization, and ventricular arrhythmias. Microarray analysis reveals elevations in transcripts of hypertrophy/remodeling genes and reductions in genes important for suppressing hypertrophy ( Hdac9) and for ventricular repolarization ( Kcnd2 ) and conduction ( Hcn4 ). These transcriptional changes are accompanied by action potential prolongation and reductions in transient outward current ( I to ; Kcnd2 ). Similarly, the pacemaker current ( I f ; Hcn4 ) is suppressed in atrioventricular nodal cells, accounting for the observed heart block. Conclusions— Trpm7 is dispensable in adult ventricular myocardium under basal conditions but is critical for myocardial proliferation during early cardiogenesis. Loss of Trpm7 at an intermediate developmental time point alters the myocardial transcriptional profile in adulthood, impairing ventricular function, conduction, and repolarization. |
| Databáze: | OpenAIRE |
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