Identification of yeast phase of pathogenic fungi by the specificity of their aminopeptidase(s)
| Autor: | K. L. Lee, Charlotte C. Campbell, Maria E. Reca, R.R. Watson |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Histoplasma
Aminopeptidases Aminopeptidase Microbiology Naphthylamine Candida albicans Animals Humans chemistry.chemical_classification Paracoccidioides brasiliensis Cryptococcus neoformans biology Blastomyces dermatitidis Sporothrix Fungi Paracoccidioides Haplorhini General Medicine biology.organism_classification Yeast Amino acid Infectious Diseases chemistry Biochemistry Blastomyces |
| Zdroj: | Medical Mycology. 13:132-141 |
| ISSN: | 1460-2709 1369-3786 |
| Popis: | Specificity of aminopeptidase(s) was fluorimetrically determined in the yeast phase of Histoplasma capsulatum, H. duboisii, H. farciminosum, Blastomyces dermatitidis, Paracoccidioides brasiliensis, Candida albicans and Crytococcus neoformans. After individually incubating each of 26 amino acid-beta-naphthylamides with each yeast, the amount of each amino acid-beta-naphthylamide hydrolyzed was determined by measuring the free naphythylamine. This resulted in a reproducible profile of the aminopeptidase(s) for each fungus when medium, growth time, size of inoculum and incubation period were standardized. This technique provided a rapid and specific means of identification and differentiation among these yeasts. Specific amino acids were identified from the profile of each yeast as those rapidly liberated by the aminopeptidase(s) of that yeast. These amino acids delineated the amino acid requirement for the normal growth of each yeast. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|