Stress-induced inhibition of translation independently of eIF2α phosphorylation
| Autor: | Cathrine A. Bøe, Per Sunnerhagen, Erik Boye, Gro Elise Rødland, Tine Weise Håland, Beáta Grallert, Jon Halvor Jonsrud Knutsen |
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| Rok vydání: | 2015 |
| Předmět: |
SPBC4B4.04
Translation Ultraviolet Rays Eukaryotic Initiation Factor-2 eIF2α Protein Serine-Threonine Kinases Biology Stress SUI2 Phosphorylation cascade Stress Physiological Schizosaccharomyces Initiation factor Protein phosphorylation Phosphorylation Ultraviolet light eIF2 Translation (biology) Cell Biology EIF2S1 Eukaryotic translation initiation factor 4 gamma Cell biology Protein Biosynthesis Schizosaccharomyces pombe Proteins Research Article |
| Zdroj: | Journal of Cell Science |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.176545 |
| Popis: | Exposure of fission yeast cells to ultraviolet (UV) light leads to inhibition of translation and phosphorylation of the eukaryotic initiation factor-2α (eIF2α). This phosphorylation is a common response to stress in all eukaryotes. It leads to inhibition of translation at the initiation stage and is thought to be the main reason why stressed cells dramatically reduce protein synthesis. Phosphorylation of eIF2α has been taken as a readout for downregulation of translation, but the role of eIF2α phosphorylation in the downregulation of general translation has not been much investigated. We show here that UV-induced global inhibition of translation in fission yeast cells is independent of eIF2α phosphorylation and the eIF2α kinase general control nonderepressible-2 protein (Gcn2). Also, in budding yeast and mammalian cells, the UV-induced translational depression is largely independent of GCN2 and eIF2α phosphorylation. Furthermore, exposure of fission yeast cells to oxidative stress generated by hydrogen peroxide induced an inhibition of translation that is also independent of Gcn2 and of eIF2α phosphorylation. Our findings show that stress-induced translational inhibition occurs through an unknown mechanism that is likely to be conserved through evolution. Summary: In contrast to textbook knowledge, the phosphorylation of translation initiation factor eIF2α is not required for UV-induced inhibition of protein synthesis, which we show in three different cell types. |
| Databáze: | OpenAIRE |
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