Genome-wide identification and expression profiling of long non-coding RNAs in auditory and vestibular systems
| Autor: | Kobi Perl, Karen B. Avraham, Kathy Ushakov, Aviv Rom, Tal Koffler-Brill, Igor Ulitsky |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Science Computational biology Biology Genome Article Mice 03 medical and health sciences medicine otorhinolaryngologic diseases Animals Inner ear Epigenetics Gene Auditory Cortex Regulation of gene expression Vestibular system Multidisciplinary Gene Expression Profiling RNA Cochlea Gene expression profiling 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Ear Inner Medicine RNA Long Noncoding Vestibule Labyrinth Transcriptome Genome-Wide Association Study |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-08320-3 |
| Popis: | Mammalian genomes encode multiple layers of regulation, including a class of RNA molecules known as long non-coding RNAs (lncRNAs). These are >200 nucleotides in length and similar to mRNAs, they are capped, polyadenylated, and spliced. In contrast to mRNAs, lncRNAs are less abundant and have higher tissue specificity, and have been linked to development, epigenetic processes, and disease. However, little is known about lncRNA function in the auditory and vestibular systems, or how they play a role in deafness and vestibular dysfunction. To help address this need, we performed a whole-genome identification of lncRNAs using RNA-seq at two developmental stages of the mouse inner ear sensory epithelium of the cochlea and vestibule. We identified 3,239 lncRNA genes, most of which were intergenic (lincRNAs) and 721 are novel. We examined temporal and tissue specificity by analyzing the developmental profiles on embryonic day 16.5 and at birth. The spatial and temporal patterns of three lncRNAs, two of which are in proximity to genes associated with hearing and deafness, were explored further. Our findings indicate that lncRNAs are prevalent in the sensory epithelium of the mouse inner ear and are likely to play key roles in regulating critical pathways for hearing and balance. |
| Databáze: | OpenAIRE |
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