Chlamydia pneumoniae-Induced Memory CD4+T-Cell Activation in Human Peripheral Blood Correlates with Distinct Antibody Response Patterns
| Autor: | Sabine Zeller, Florian Kern, Sebastian Bunk, Hanne Schaffert, Marina Borisova, Bianca Schmid, Jan Rupp, Corinna Hermann, Christoph Goletz |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Microbiology (medical) Interleukin 2 Clinical Biochemistry Immunology medicine.disease_cause Peripheral blood mononuclear cell Microbiology Interferon-gamma Immune system Bacterial Proteins Antigen medicine Humans Immunology and Allergy Interferon gamma CD154 Cells Cultured Antigens Bacterial biology DNA-Directed RNA Polymerases Chlamydophila pneumoniae Middle Aged Antibodies Bacterial Blood Leukocytes Mononuclear biology.protein Interleukin-2 Female Microbial Immunology Antibody Molecular Chaperones medicine.drug |
| Zdroj: | Clinical and Vaccine Immunology. 17:705-712 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cvi.00209-09 |
| Popis: | Chlamydia pneumoniaeis a frequent pathogen of the respiratory tract, and persistent infections with this obligate intracellular bacterium have been associated with different severe sequelae. Although T-cell activation during acuteC. pneumoniaeinfections has been described, little is known about the frequency or the role of theC. pneumoniae-specific memory T cells that reside in the human body after the resolution of the infection. In the present study, theC. pneumoniae-induced T-cell responses in peripheral blood mononuclear cells of 56 healthy volunteers were analyzed and compared to the donor's serum antibody reactivity toward wholeC. pneumoniaeas well as recombinantC. pneumoniaeantigens. Following short-term stimulation withC. pneumoniae, both gamma interferon (IFN-γ)- and interleukin-2 (IL-2)-producing CD4+T-cell responses could be detected in 16 of 56 healthy individuals.C. pneumoniae-activated CD4+T cells expressed CD154, a marker for T-cell receptor-dependent activation, and displayed a phenotype of central memory T cells showing dominant IL-2 production but also IFN-γ production. Interestingly, individuals with both IFN-γ- and IL-2-producing responses showed significantly decreased immunoglobulin G reactivity towardC. pneumoniaeRpoA and DnaK, antigens known to be strongly upregulated during chlamydial persistence, compared to IgG reactivity of seropositive individuals with no T-cell response or CD4+T-cell responses involving the production of a single cytokine (IFN-γ or IL-2). Our results demonstrate that memory CD4+T cells responding toC. pneumoniaestimulation can be detected in the circulation of healthy donors. Furthermore, among seropositive individuals, the presence or the absence of dual IFN-γ- and IL-2-producing T-cell responses was associated with distinct patterns of antibody responses toward persistence-associatedC. pneumoniaeantigens. |
| Databáze: | OpenAIRE |
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