The Immunogenicity and Protective Efficacy of Bovine Herpesvirus 1 Glycoprotein D plus Emulsigen Are Increased by Formulation with CpG Oligodeoxynucleotides
| Autor: | P. Griebel, Rolf Hecker, S. van Drunen Littel-van den Hurk, X. P. Ioannou, Lorne A. Babiuk |
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| Rok vydání: | 2002 |
| Předmět: |
CpG Oligodeoxynucleotide
Immunology Cattle Diseases Antibodies Viral Lymphocyte Activation Microbiology Virus Viral Proteins Immune system Adjuvants Immunologic Neutralization Tests T-Lymphocyte Subsets Immunity Virology Vaccines and Antiviral Agents Animals Herpesvirus 1 Bovine Vaccines Synthetic biology Immunogenicity Viral Vaccines hemic and immune systems Herpesviridae Infections respiratory system biology.organism_classification Molecular biology Bovine herpesvirus 1 Oligodeoxyribonucleotides CpG site Insect Science biology.protein Cattle CpG Islands Antibody |
| Zdroj: | Journal of Virology. 76:9002-9010 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.76.18.9002-9010.2002 |
| Popis: | The immunogenicity and protective efficacy of a bovine herpesvirus 1 (BHV-1) subunit vaccine formulated with Emulsigen (Em) and a synthetic oligodeoxynucleotide containing unmethylated CpG dinucleotides (CpG ODN) was determined in cattle. A truncated, secreted version of BHV-1 glycoprotein D (tgD) formulated with Em and CpG ODN at concentrations of 25, 2.5, or 0.25 mg/dose produced a more balanced immune response, higher levels of virus neutralizing antibodies, and greater protection after BHV-1 challenge compared to tgD adjuvanted with either Em or CpG ODN alone. In contrast, tgD formulated with Em and either 25 mg of a non-CpG ODN or another immunostimulatory compound, dimethyl dioctadecyl ammonium bromide, induced similar immunity and protection compared to tgD formulated with Em alone, a finding which confirms the immunostimulatory effect of ODN to be CpG motif mediated. Our results demonstrate the ability of CpG ODN to induce a strong and balanced immune response in a target species. |
| Databáze: | OpenAIRE |
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