Cationic polyelectrolyte copolymer modified polyurethane foam for flow injection preconcentration and separation of trace amounts of β-lactam antibiotics
| Autor: | Mohamed F. El-Shahat, Heinz-Martin Kuss, S.M. Abdel Azeem |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Cefotaxime
Polymers Polyurethanes Chemie beta-Lactams Analytical Chemistry chemistry.chemical_compound medicine Hydroxides Animals Humans Solid phase extraction Flow injection analysis Detection limit Chromatography Chemistry Solid Phase Extraction Cationic polymerization Chloranil Analytic Sample Preparation Methods Hydrogen-Ion Concentration Polyelectrolyte Anti-Bacterial Agents Milk Flow Injection Analysis Lactam Chlorine medicine.drug |
| Popis: | A more sensitive flow injection preconcentration method has been developed for the determination of four β-lactam antibiotics (BLAs) namely cefaclor, cefotaxime, amoxicillin and ampicillin in urine, pharmaceuticals and milk. A mini-column packed with PUF functionalized with the cationic polyelectrolyte, poly( N -chloranil N , N , N ′, N ′-tetramethylethylene diammonium dichloride) PCTDD, was utilized for selective preconcentration. The detection limits with this method were 3.3, 3.8, 5.1 and 7.0 ng mL −1 and enrichment factors were 38, 21, 39, and 36 for cefaclor, cefotaxime, amoxicillin and ampicillin, respectively with a sample throughput of 12 h −1 for all BLAs. Moreover, the BLAs were successfully separated by isocratic elution using a micellar mobile phase. Application of the method developed has resulted in recovery values in the range 95%–109% ( RSD ≤8.7), 83%–99% ( RSD ≤9.7) and 91%–103% ( RSD ≤4.0) for urine, pharmaceuticals and milk samples, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|