A Rapid and Reliable Assay for Regioselectivity Using Fluorescence Spectroscopy

Autor:	Goran Angelovski, Stefan Grudzielanek, Mark D. Keränen, Petra Linnepe, Peter Eilbracht
Rok vydání:	2006
Předmět:	Rapid acquisition Chromatography Tandem Chemistry Fischer indole synthesis Analytical chemistry Structural isomer Regioselectivity General Chemistry Fluorescence Fluorescence spectroscopy Hydroformylation
Zdroj:	Advanced Synthesis & Catalysis
ISSN:	1615-4169 1615-4150
DOI:	10.1002/adsc.200606047
Popis:	The first report of a fluorescence-based assay for the direct measurement of the regioselectivity of a reaction is described herein, developed from the desire to construct a quicker analytical method for the determination of the ratio of regioisomers obtained in the tandem hydroformylation/Fischer indole synthesis. The method allows for extremely rapid acquisition times, as the use of crude reaction mixtures is allowed. The assay is also shown to be overall very reliable, tolerating the presence of various functional groups and proceeding on average with a standard error of measurement comparable to that of NMR. As fluorescence is the only requirement for the employment of this analytical method, countless numbers of target-specific assays can undoubtedly be developed based upon this initial finding.
Databáze:	OpenAIRE
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