Bar-HRM for identification of cryptic earthworm species
| Autor: | Lise Dupont, Gaspard Baudrin, Virginie Roy, Agnès Gigon |
|---|---|
| Přispěvatelé: | Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris ), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Mitochondrial DNA biology Sentinel species [SDV]Life Sciences [q-bio] Earthworm Soil Science Allolobophora chlorotica 04 agricultural and veterinary sciences biology.organism_classification 010603 evolutionary biology 01 natural sciences DNA barcoding High Resolution Melt Taxon Evolutionary biology 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Identification (biology) Ecology Evolution Behavior and Systematics |
| Zdroj: | Pedobiologia Pedobiologia, Elsevier, 2020, 80, pp.150634-. ⟨10.1016/j.pedobi.2020.150634⟩ Pedobiologia, 2020, 80, pp.150634-. ⟨10.1016/j.pedobi.2020.150634⟩ |
| ISSN: | 0031-4056 |
| DOI: | 10.1016/j.pedobi.2020.150634⟩ |
| Popis: | Accurate species identification is crucial in ecological research on sentinel species such as earthworms. Here, we developed a reliable and cost-efficient method for the rapid identification of taxa within the Allolobophora chlorotica complex of cryptic earthworm species. We combined high resolution melting (HRM) analysis with DNA barcoding (Bar-HRM) in a three-step approach: (i) selection of a mini-barcode in the 16S mitochondrial gene, (ii) test of the method on a panel of 16 reference individuals and (iii) identification at the species level of 24 unknown specimens. Thus, we proved the efficacy of Bar-HRM to discriminate between cryptic sister (or sibling) earthworm species and we provided a standardized method that can be easily adapted to other taxa. |
| Databáze: | OpenAIRE |
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