High growth reassortant influenza vaccine viruses: new approaches to their control
| Autor: | John Wood, R.W. Newman, Diane Major, U. Dunleavy, Carolyn Nicolson, James S. Robertson |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Virus Cultivation
Influenza vaccine Orthomyxoviridae Molecular Sequence Data Bioengineering Applied Microbiology and Biotechnology Polymerase Chain Reaction Virus law.invention Vaccine strain law Humans Influenzavirus Gene Antigens Viral Polymerase chain reaction Pharmacology Recombination Genetic General Immunology and Microbiology biology Base Sequence Strain (biology) General Medicine biology.organism_classification Virology Influenza B virus Phenotype Influenza A virus Influenza Vaccines DNA Viral Oligonucleotide Probes Biotechnology |
| Zdroj: | Biologicals : journal of the International Association of Biological Standardization. 20(3) |
| ISSN: | 1045-1056 |
| Popis: | When a new strain of an influenza virus is required to be incorporated into influenza vaccine, attempts are made to recombine such strains with laboratory adapted viruses, which will grow to high titre in order to improve the yield of the vaccine strain. It is important that such high growth reassortant vaccine strains are not contaminated with genes coding for the antigenic determinants of the high growth laboratory strain. We describe the characterization of two recent high growth reassortants and the application of the polymerase chain reaction to ensure their genetic identity and purity. |
| Databáze: | OpenAIRE |
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