Ultrasound and Microbubble-Targeted Delivery of Small Interfering Rna Into Primary Endothelial Cells Is More Effective Than Delivery of Plasmid Dna
| Autor: | Robert H. Henning, Lynda J.M. Juffermans, Bernadet D. M. Meijering, Leo E. Deelman |
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| Přispěvatelé: | Cardiology, ICaR - Ischemia and repair, Groningen Kidney Center (GKC), Vascular Ageing Programme (VAP), Groningen Institute for Organ Transplantation (GIOT) |
| Rok vydání: | 2014 |
| Předmět: |
Small interfering RNA
LOADED MICROBUBBLES Acoustics and Ultrasonics Pore formation Swine ATTENUATES NEOINTIMAL FORMATION Transgene Endothelial cells ENDOCYTOSIS Sulfur Hexafluoride Biophysics MEDIATED GENE DELIVERY Endocytosis Transfection Clathrin ANGIOGENESIS High-Energy Shock Waves Sonication SIRNA DELIVERY Caveolin Animals Radiology Nuclear Medicine and imaging RNA Small Interfering OPTIMIZATION Cells Cultured Phospholipids Glyceraldehyde 3-phosphate dehydrogenase DESTRUCTION Microbubbles Radiological and Ultrasound Technology biology Chemistry Ultrasound and microbubbles ARTERIAL INJURY Plasmid DNA Virology Molecular biology Electroporation Cytoplasm biology.protein SKELETAL-MUSCLE Plasmids |
| Zdroj: | Ultrasound in Medicine and Biology, 40(3), 532-540. Elsevier USA Juffermans, L J M, Meijering, B D M, Henning, R H & Deelman, L E 2014, ' Ultrasound and Microbubble-Targeted Delivery of Small Interfering Rna Into Primary Endothelial Cells Is More Effective Than Delivery of Plasmid Dna ', Ultrasound in Medicine and Biology, vol. 40, no. 3, pp. 532-540 . https://doi.org/10.1016/j.ultrasmedbio.2013.09.031 Ultrasound in Medicine and Biology, 40(3), 532-540. ELSEVIER SCIENCE INC |
| ISSN: | 0301-5629 |
| DOI: | 10.1016/j.ultrasmedbio.2013.09.031 |
| Popis: | Ultrasound and microbubble-targeted delivery (UMTD) is a promising non-viral technique for genetic-based therapy. We found that UMTD of small interfering RNA (siRNA) is more effective than delivery of plasmid DNA (pDNA). UMTD (1 MHz, 0.22 MPa) of fluorescently labeled siRNA resulted in 97.9 +/- 1.5% transfected cells, with siRNA localized homogenously in the cytoplasm directly after ultrasound exposure. UMTD of fluorescently labeled pDNA resulted in only 43.0 +/- 4.2% transfected cells, with localization mainly in vesicular structures, co-localizing with endocytosis markers clathrin and caveolin. Delivery of siRNA against GAPDH (glyceraldehyde-3phosphate dehydrogenase) effectively decreased protein levels to 24.3 +/- 7.9% of non-treated controls (p |
| Databáze: | OpenAIRE |
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