Evaluation of current derivative spectrophotometric methodology for the determination of percent carboxyhemoglobin saturation in postmortem blood samples
| Autor: | B.J. Perrigo, Brian P. Joynt |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Chemical Health and Safety
Chromatography Chromatography Gas Time Factors Test procedures Swine Health Toxicology and Mutagenesis Uv spectrum Carboxyhemoglobin levels Postmortem blood Carbon monoxide intoxication Toxicology Analytical Chemistry Specimen Handling chemistry.chemical_compound chemistry Carboxyhemoglobin Environmental Chemistry Animals Analytical procedures Spectrophotometry Ultraviolet Autopsy Saturation (chemistry) |
| Zdroj: | Journal of analytical toxicology. 13(1) |
| ISSN: | 0146-4760 |
| Popis: | I Carbon monoxide intoxication continues to be a commonly encountered cause of death in most areas of Canada. The forensic nature of the samples in these cases presents special problems that are not normally encountered in clinical determinations. A study was undertaken to assess various methods of determining the percent carboxyhemoglobin saturation in blood, more specifically, those using derivative spectrophotometric measurements In the Soret region of the UV spectrum. At the same time, other studies were carried out: the effects of storage time on the carboxyhemoglobin levels; evaluation of sample containers; comparison of percent carboxyhemoglobin saturation in blood samples taken ante-mortem and post-mortem. Blood for the study was obtained from laboratory animals that were exposed to carbon monoxide before death. The approach taken in our study was to examine the perfor- mance of a proposed method by using pig "autopsy" blood samples. The opportunity arose to sacrifice pigs under the effect of CO gas and to monitor the experimental results of various analytical procedures during lengthy periods of storage. It was felt that sacrifice of these animals and subsequent, collection of blood under standard autopsy conditions would afford an op- portunity to test procedures without some of the uncertainties of past work in this field. The proposed method combines elements of procedures pub- lished by Rodkey (9), Beutler (10), Fukui (2), and Parks (3). A wavelength monitoring technique similar to that described by Taulier (5) was also incorporated. Blood samples were also an- alyzed by the gas chromatographic procedures of Kupferschmidt (11); however, sampling was limited because this procedure re- quired a larger volume of blood. |
| Databáze: | OpenAIRE |
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