Lack of molecular evidence of fecal-borne viruses in capybaras from São Paulo state, Brazil, 2018-2020: a minor public health issue

Autor: Lais Sampaio de Azevedo, Yasmin França, Ellen Viana, Roberta Salzone Medeiros, Simone Guadagnucci Morillo, Raquel Guiducci, Cibele Daniel Ribeiro, Heloisa Rosa Vieira, Karolina Morales Barrio-Nuevo, Mariana Sequetin Cunha, Juliana Mariotti Guerra, Dulcilena de Matos Castro e Silva, Valter Batista Duo Filho, Emerson Luiz Lima Araújo, Sérgio Roberto Santos Ferreira, Camila Freitas Batista, Gislaine Celestino Dutra da Silva, Maurício Lacerda Nogueira, Cintia Mayumi Ahagon, Regina Célia Moreira, Lia Cunha, Vanessa Santos Morais, Antonio Charlys da Costa, Adriana Luchs
Rok vydání: 2022
Předmět:
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].
ISSN: 1678-4405
Popis: Capybara (Hydrochoerus hydrochaeris) is the world's largest rodent species distributed throughout South America. These animals are incredibly tolerant to anthropogenic environments and are occupying large urban centers. Capybaras are known to carry potentially zoonotic agents, including R. rickettsia, Leishmania spp., Leptospira spp., Trypanosoma spp., Salmonella spp., Toxoplasma gondii, and rabies virus. Focusing on the importance of monitoring potential sources of emerging zoonotic viruses and new viral reservoirs, the aim of the present study was to assess the presence of fecal-borne viruses in the feces of capybaras living in urban parks in São Paulo state, Brazil. A total of 337 fecal samples were collected between 2018 and 2020 and screened for the following: (i) Rotavirus group A (RVA) by ELISA; (ii) non-RVA species and Picobirnavirus (PBV) using PAGE; (iii) Human Bocaparvovirus (HBoV), Bufavirus (BuV), Tusavirus (TuV), and Cutavirus (CuV) qPCR; (iv) Human Enterovirus (EV), Norovirus GII (NoV), and Hantavirus by in houses RT-qPCR; (v) SARS-CoV-2 via commercial RT-qPCR kit assay; and (vi) Astrovirus (AstV) and Adenovirus (AdV) using conventional nested (RT)-PCRs. All fecal samples tested were negative for fecal-borne viruses. This study adds further evidence that the fecal-borne viruses is a minor public health issue in Brazilian capybaras, at least during the surveillance period and surveyed areas. Continuous monitoring of sylvatic animals is essential to prevent and control the emergence or re-emergence of newly discovered virus as well as viruses with known zoonotic potential.
Databáze: OpenAIRE
Externí odkaz: