Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance
| Autor: | Luis Gustavo Romani Fernandes, Patricia Ucelli Simioni, Wirla Maria da Silva Cunha Tamashiro, D. L. Gabriel, Aureo T. Yamada, Maristela Ruberti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
CD4-Positive T-Lymphocytes Male Article Subject Ovalbumin Immunology Administration Oral Mice Transgenic chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes environment and public health digestive system Immune tolerance Interferon-gamma Mice Intestinal mucosa Antigens CD T-Lymphocyte Subsets Transforming Growth Factor beta Intestine Small Immune Tolerance Immunology and Allergy Medicine Animals IL-2 receptor Lymphocyte Count Mice Inbred BALB C business.industry Tumor Necrosis Factor-alpha FOXP3 Epithelial Cells hemic and immune systems General Medicine Small intestine medicine.anatomical_structure Intraepithelial lymphocyte Tumor necrosis factor alpha business lcsh:RC581-607 CD8 Injections Intraperitoneal Research Article |
| Zdroj: | Clinical and Developmental Immunology Clinical and Developmental Immunology, Vol 2012 (2012) |
| ISSN: | 1740-2522 |
| DOI: | 10.1155/2012/208054 |
| Popis: | In this work, we evaluated the effects of administration of OVA on phenotype and function of intraepithelial lymphocytes (IELs) from small intestine of transgenic (TGN) DO11.10 and wild-type BALB/c mice. While the small intestines from BALB/c presented a well preserved structure, those from TGN showed an inflamed aspect. The ingestion of OVA induced a reduction in the number of IELs in small intestines of TGN, but it did not change the frequencies of CD8+and CD4+T-cell subsets. Administration of OVA via oral + ip increased the frequency of CD103+cells in CD4+T-cell subset in IELs of both BALB/c and TGN mice and elevated its expression in CD8β+T-cell subset in IELs of TGN. The frequency of Foxp3+cells increased in all subsets in IELs of BALB/c treated with OVA; in IELs of TGN, it increased only in CD25+subset. IELs from BALB/c tolerant mice had lower expression of all cytokines studied, whereas those from TGN showed high expression of inflammatory cytokines, especially of IFN-γ, TGF-β, and TNF-α. Overall, our results suggest that the inability of TGN to become tolerant may be related to disorganization and altered proportions of inflammatory/regulatory T cells in its intestinal mucosa. |
| Databáze: | OpenAIRE |
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