Preclinical Diagnosis of African Swine Fever in Contact-Exposed Swine by a Real-Time PCR Assay
| Autor: | Laszlo Zsak, G. R. Risatti, Richard A. French, Johnny D. Callahan, Z. Lu, Manuel V. Borca, Gerald F. Kutish, A. Zsak, W. M. Nelson, Daniel L. Rock, J. G. Neilan |
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| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Swine Palatine Tonsil Polymerase Chain Reaction Sensitivity and Specificity African swine fever virus law.invention law Virology TaqMan medicine Animals Taq Polymerase African Swine Fever Polymerase chain reaction biology Hybridization probe Outbreak biology.organism_classification African Swine Fever Virus Real-time polymerase chain reaction medicine.anatomical_structure Tonsil Viral disease DNA Probes |
| Zdroj: | Journal of Clinical Microbiology. 43:112-119 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak. |
| Databáze: | OpenAIRE |
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